Alternative Polyadenylation:Methods, Findings, and Impacts
- Author:
Chen WEI
1
;
Jia QI
;
Song YIFAN
;
Fu HAIHUI
;
Wei GANG
;
Ni TING
Author Information
1. State Key Laboratory of Genetic Engineering&MOE Key Laboratory of Contemporary Anthropology
- Keywords:
Alternative polyadenylation;
Next-generation sequencing;
30UTR;
Alternative splicing;
Gene regulation
- From:
Genomics, Proteomics & Bioinformatics
2017;15(5):287-300
- CountryChina
- Language:Chinese
-
Abstract:
Alternative polyadenylation (APA), a phenomenon that RNA molecules with different 30 ends originate from distinct polyadenylation sites of a single gene, is emerging as a mechanism widely used to regulate gene expression. In the present review, we first summarized various methods prevalently adopted in APA study, mainly focused on the next-generation sequencing (NGS)-based techniques specially designed for APA identification, the related bioinformatics methods, and the strategies for APA study in single cells. Then we summarized the main findings and advances so far based on these methods, including the preferences of alternative polyA (pA) site, the biological processes involved, and the corresponding consequences. We especially categorized the APA changes discovered so far and discussed their potential functions under given conditions, along with the possible underlying molecular mechanisms. With more in-depth studies on extensive samples, more signatures and functions of APA will be revealed, and its diverse roles will gradually heave in sight.
