Protective effects of epigallocatechin gallate on homocysteine-induced proliferation of vascular smooth muscle cells
10.7619/jcmp.201713005
- VernacularTitle:表没食子儿茶素没食子酸酯对同型半胱氨酸促血管平滑肌细胞增殖的保护作用
- Author:
Xiaoli ZHAN
1
;
Dongchi ZHOU
;
Huifang GU
;
Xiuhong YANG
;
Huimin JIN
Author Information
1. 上海市浦东医院 肾脏内科
- Keywords:
homocysteine;
epigallocatechin gallate;
proliferation;
angiotensinII;
angiotensin Ⅱ type 1 receptor
- From:
Journal of Clinical Medicine in Practice
2017;21(13):17-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of epigallocatechin gallate(EGCG)on homocysteine-induced proliferation of human aortic smooth muscle cells(HASMCs).Methods HASMCs were served as objects,which were cultured in various media,including Hcy(100,200,500,1 000 μmol/L),EGCG(EGCG 5,10,20 μmol/L),Hcy+EGCG(Hcy 500 μmol/L+EGCG 5 μmol/L,Hcy 500 μmol/L+EGCG 10 μmol/L,Hcy 500 μmol/L+EGCG 20 μmol/L)for 24 h.The proliferation of HASMCs was determined by cell counting kit-8(CCK-8)and bromodeoxyuridine(BrdU)labeling.The level of angiotensin Ⅱ(AngII)was determined by enzyme-linked immunosorbent assay(ELISA),and the level of angiotensin Ⅱ type 1 receptor(AT-1R)was determined by Western blot.Results After treatment for 24 h,compared with the control group,the proliferation of cells in different Hcy groups significantly increased(P<0.05),while the proliferation of cells in EGCG 10 and 20 μmol/L group significantly reduced(P<0.05).Compared with the Hcy 500 μmol/L group,cell proliferation significantly reduced after adding EGCG 10 and 20 μmol/L(P<0.01),while the expression levels of AngII and AT-1R decreased significantly(P<0.01).Conclusion EGCG can inhibit Hcy-induced proliferation of VSMCs.
