Effects of EGFR interference on activation of astrocytes after intra-cerebral hemorrhage and its mechanism
10.7619/jcmp.201713004
- VernacularTitle:干扰表皮生长因子受体表达对脑出血后星形胶质细胞活化的影响及其机制
- Author:
Zhengbin WU
1
;
Dalin LUO
;
Dongpo JIANG
Author Information
1. 重庆市东南医院 ICU
- Keywords:
epidermal growth factor;
glial fibrillary acidic protein;
phosphorylated tyrosine kinase JAK1;
phosphorylated signal transducer and activator of transcription 3;
astrocytes
- From:
Journal of Clinical Medicine in Practice
2017;21(13):12-16,21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of epidermal growth factor receptor(EGFR)interference on activation of astrocytes after intra-cerebral hemorrhage and its mechanism.Methods Rat model of intra-cerebral hemorrhage was induced by injection of Ⅶ collagenase.Sham operation group was set as control.After 7 d,the neurological function score was used to judge whether the model was successful or not.The expression of EGFR and GFAP in brain tissue of rats in intra-cerebral hemorrhage group and sham operation group were detected by SP immunohistochemical method.Astrocytes were isolated from newborn rats' cerebral cortex and cultured in vivo.Effects of EGFR interference on activation of astrocytes were observed.The experimental rats were divided into 4 groups: ① control group was not handled.② CNTF group was added with 20 μg/L CNTF.③ Negative group was added with 20 μg/L CNTF,and EGFR siRNA negative control was transfected.④ EGFR group was added with 20 μg/L CNTF,and EGFR siRNA was transfected.The expressions of EGFR,GFAP,p-JAK1,p-STAT3 were detected by real-time PCR and Western blot.Results The modeling success rate was 89.5％(17/19).The expressions of EGFR and GFAP in brain tissue of rats with intra-cerebral hemorrhage were significantly higher than those in sham operation group(P<0.05).The relative expressions of EGFR mRNA and protein in EGFR group were significantly lower than those in control group,CNTF group and negative group(P<0.05).The relative expressions of GFAP mRNA and protein in the CNTF group and the negative group were significantly higher than those in the controlgroup and the EGFR group(P<0.05),but there was no significant difference between the control group and the EGFR group(P>0.05).The relative expressions of p-JAK1 and p-STAT3 protein in the CNTF group and the negative group were significantly higher than those in the control group and the EGFR group(P<0.05),but there was no significant difference between the control group and the EGFR group(P>0.05).Conclusion The expressions of EGFR and GFAP significantly increase in the rats with intra-cerebral hemorrhage.Interference of EGFR expression can inhibit the activation of astrocytes in rats,which may be related with the inhibition of JAK1 and STAT3 phosphorylation.
