Correlating Bladder Cancer Risk Genes with Their Targeting MicroRNAs Using MMiRNA-Tar
- Author:
Liu YANG
1
;
Baker STEVE
;
Jiang HUI
;
Stuart GARY
;
Bai YONGSHENG
Author Information
1. Department of Electrical and Computer Engineering
- Keywords:
The Cancer Genome Atlas;
Bladder cancer;
MicroRNA;
mRNA;
Correlation;
Target prediction
- From:
Genomics, Proteomics & Bioinformatics
2015;(3):177-182
- CountryChina
- Language:Chinese
-
Abstract:
The Cancer Genome Atlas (TCGA) (http://cancergenome.nih.gov) is a valuable data resource focused on an increasing number of well-characterized cancer genomes. In part, TCGA provides detailed information about cancer-dependent gene expression changes, including changes in the expression of transcription-regulating microRNAs. We developed a web interface tool MMiRNA-Tar (http://bioinf1.indstate.edu/MMiRNA-Tar) that can calculate and plot the correlation of expression for mRNA?microRNA pairs across samples or over a time course for a list of pairs under different prediction confidence cutoff criteria. Prediction confidence was estab-lished by requiring that the proposed mRNA?microRNA pair appears in at least one of three target prediction databases:TargetProfiler, TargetScan, or miRanda. We have tested our MMiRNA-Tar tool through analyzing 53 tumor and 11 normal samples of bladder urothelial carcinoma (BLCA) datasets obtained from TCGA and identified 204 microRNAs. These microRNAs were correlated with the mRNAs of five previously-reported bladder cancer risk genes and these selected pairs exhib-ited correlations in opposite direction between the tumor and normal samples based on the cus-tomized cutoff criterion of prediction. Furthermore, we have identified additional 496 genes (830 pairs) potentially targeted by 79 significant microRNAs out of 204 using three cutoff criteria, i.e.
