Simultaneous determination for metabolites of benzene compounds in urine by high performance liquid chromatography
10.3760/cma.j.cn121094-20190909-00373
- VernacularTitle:尿中7种苯系物代谢产物测定的实时肌酐校正法
- Author:
Mengjia HUANG
1
;
Yue SHEN
;
Kunpeng MA
Author Information
1. 200052 上海，上海交通大学附属上海市胸科医院
- Keywords:
Benzene;
Metabolites;
High performance liquid chromatography;
Urine;
Creatinine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(3):213-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To describe for the determination of contents of metabolites of benzene compounds in urine sample by high performance liquid chromatography.Methods:After acidification with hydrochloric acid, metabolites in urine were first extracted by acetonitrile and isopropanol (V∶V, 9∶1) with excessive sodium chloride, then gradient separated on a C18 column and then determined by DAD detector.Results:There were good linear relationship between peak areas and injection quality in range of 2.00-100 mg/L ( r>0.999). The detection limit and quantitative limit of this method were 4.15-70.7 μg/L and 13.8-235 μg/L respectively. The precision for the analysis of urine was1.78%-8.23% ( n =6). The average recovery of metabolites was 85.4%-105.5% at thee spiked levels in the range of 2.00-100 mg/L. Conclusion:The accuracy and reproducibility obtained make this method useful for the biological monitoring of occupational exposure to toluene, xylene, styrene and ethylbenzene.
