Complete Sequence of pABTJ2, A Plasmid from Acinetobacter baumannii MDR-TJ, Carrying Many Phage-like Elements
- Author:
Huang HE
1
;
Dong YAN
;
Yang ZHI-LIANG
;
Luo HAO
;
Zhang XI
;
Gao FENG
Author Information
1. M0E Key Laboratory of Systems Bioengineering
- Keywords:
A. baumannii;
Plasmid;
tRNA gene;
Phage-like elements
- From:
Genomics, Proteomics & Bioinformatics
2014;(4):172-177
- CountryChina
- Language:Chinese
-
Abstract:
Acinetobacter baumannii is an important opportunistic pathogen in hospital, and the multidrug-resistant isolates of A. baumannii have been increasingly reported in recent years. A num-ber of different mechanisms of resistance have been reported, some of which are associated with plasmid-mediated acquisition of genes. Therefore, studies on plasmids in A. baumannii have been a hot issue lately. We have performed complete genome sequencing of A. baumannii MDR-TJ, which is a multidrug-resistant isolate. Finalizing the remaining large scaffold of the previous assem-bly, we found a new plasmid pABTJ2, which carries many phage-like elements. The plasmid pAB-TJ2 is a circular double-stranded DNA molecule, which is 110,967 bp in length. We annotated 125 CDSs from pABTJ2 using IMG ER and ZCURVE_V, accounting for 88.28%of the whole plasmid sequence. Many phage-like elements and a tRNA-coding gene were detected in pABTJ2, which is rarely reported among A. baumannii. The tRNA gene is specific for asparagine codon GTT, which may be a small chromosomal sequence picked up through incorrect excision during plasmid forma-tion. The phage-like elements may have been acquired during the integration process, as the GC content of the region carrying phage-like elements was higher than that of the adjacent regions. The finding of phage-like elements and tRNA-coding gene in pABTJ2 may provide a novel insight into the study of A. baumannii pan-plasmidome.
