Expression of p53 protein, PCNA, and Ki-67 in osteosarcomas of bone.
10.3346/jkms.1995.10.5.360
- Author:
Hye Rim PARK
1
;
Yong Koo PARK
Author Information
1. Department of Pathology, College of Medicine, Hallym University, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
p53 protein;
PCNA;
Ki-67;
Immunohistochemistry;
Osteosarcoma
- MeSH:
Adolescent;
Adult;
Aged;
Antibodies, Monoclonal;
Bone Neoplasms/*chemistry/genetics/pathology;
Cell Cycle/physiology;
Child;
Child, Preschool;
Genes, p53;
Human;
Immunohistochemistry;
Ki-67 Antigen;
Male;
Middle Age;
Mutation;
Neoplasm Proteins/*analysis;
Nuclear Proteins/*analysis;
Osteosarcoma/*chemistry/genetics/pathology;
Proliferating Cell Nuclear Antigen/*analysis;
Protein p53/*analysis;
Support, Non-U.S. Gov't
- From:Journal of Korean Medical Science
1995;10(5):360-367
- CountryRepublic of Korea
- Language:English
-
Abstract:
Expressions of p53 protein, a product of the tumor suppressor gene were studied in osteosarcomas relating to various prognostic factors. Thirty-four osteosarcomas were investigated immunohistochemically with a monoclonal antibody clone PAb240, which recognizes a common conformational epitope of mutant p53 proteins and another clone PAb1801, which reacts with both wild- and mutant-type p53 proteins. The results were compared with expressions of proliferating cell nuclear antigen (PCNA) and Ki-67 providing a simple method for the assessment of growth fractions of tumors. PAb240 stained nuclei and cytoplasm of tumor cells in 8 of 34 osteosarcomas (23.5%), whereas PAb1801 reacted in all 34 osteosarcomas (100%). Fifteen tumors (44.1%) showed positivity for PAb1801 in more than half of the tumor cells. Twelve patients were alive and thirteen were dead. Tumors from 9 patients (75%) who survived revealed only focal positive immunoreactions with PAb1801 and tumors from 6 patients (46.1%) who died revealed diffuse reactions. Twelve cases (35.3%) showed a high PCNA index (> 40%) and fibroblastic osteosarcomas revealed the highest PCNA positivity. Twenty-two cases (64.7%) revealed a very low Ki-67 index (less than 10%) and Ki-67 index showed a good correlation with PCNA positivity (r = 0.6247). Expressions of both wild-and mutant-type p53 protein, PCNA, and Ki-67 were not correlated with other clinical or pathological parameters.
