Effect of bone marrow mesenchymal stem cells modified by GDNF in transplantation treatment of patients with spinal cord injury
10.7619/jcmp.201709024
- VernacularTitle:胶质细胞源性神经营养因子修饰的骨髓间充质干细胞移植治疗脊髓损伤的效果
- Author:
Lang WU
1
;
Cheng HUANG
;
Xinming FENG
;
Songchao BI
;
Tao CHEN
;
Peng WANG
;
Jiandong YANG
Author Information
1. 扬州大学临床医学院
- Keywords:
spinal cord injury;
bone marrow mesenchymal stem cells;
Gial cell line-derived neurotrophic factor
- From:
Journal of Clinical Medicine in Practice
2017;21(9):94-98,102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effectiveness of transplantation of neuron-like cells induced by GDNF from BMSCs on treatment of spinal cord injury in rats.Methods Transfected BMSCs by recombinant adenovirus containing the gene of rGDNF and GFP.36 SD rats with spinal cord injury were randomly divided into three groups.All the 36 SD rats with spinal cord injury were scored by the Basso,Beattle,Bresnahan locomotoRrating scale at different time points (1、2、3、4 weeks afteRoperation).In the 4th week afteRoperation,tissue from the injury section was prepared to observe repaiRof spinal cord injury.Results BMSCs was able to secrete GDNF continuously afteRtransfected by recombinant adenovirus containing the gene of rGDNF and GFP.AfteRoperation,BBB scores in intralesional treatment group significantly increased when compared with that of intravenous treatment group and injury control group.In the 4th week afteRoperation,HEmatoxylin-eosin staing results showed that cavitation in intravenous treatment group was largeRthan that in intralesional treatment group with the one of injury control group being the biggest.Immunohistochemical method showed that there were more NF200+cells and GFAP+cells in intralesional treatment group than in the otheRtwo groups,and injury control group had the least NF200+cells and GFAP+ cells.Conclusion AfteRinduced into nuron-like cells,BMSCs can treat spinal cord injury effectively.
