Relationship between urinary polycyclic aromatic hydrocarbon metabolite and cell cycle of lymphocyte in coke oven workers
10.3760/cma.j.issn.1001-9391.2016.11.004
- VernacularTitle:焦炉作业工人外周血淋巴细胞周期分布与尿中多环芳烃代谢物的关系
- Author:
Baolong PAN
1
,
2
;
Huitao ZHANG
;
Hongjie ZHANG
;
Wentao CHEN
;
Jin YANG
Author Information
1. 030001 太原 山西医科大学公共卫生学院劳动卫生学教研室
2. 太原钢铁(集团)有限公司总医院
- Keywords:
Hydrocarbons aromatic;
Cell cycle;
1-Hydroxypyrene
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(11):814-818
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between urinary polycyclic aromatic hydrocarbon metabolite and cell cycle of lymphocyte in coke oven workers.Methods 437 coke oven workers and 163 workers in water treatment department were recruited in this study.Flow cytometry was used to detect the cell cycle of lymphocyte.For the measurement of urinary metabolites,urine samples were treated with β-glucuronidase and analyzed using HPLC with a fluorescence detector.Results The concentrations of urinary 2-naphthol,2-hydroxyfluorene,9-phenanthrol and 1-hydroxypyrene 1 in coke oven workers were significantly higher than those in control group (P＜0.01).The distributions of cell cycle were analyzed in high exposure group (the content of urinary metabolites high than P75) and low exposure group (the content of urinary metabolites low than P25).According to the content of 1-hydroxypyrene,the proportions of S phase in high exposure group were significantly higher than those of low exposure group (Z=-2.496,P=0.013),but the proportions of G0/G1 phase were significantly lower than low exposure group (Z=-2.074,P=0.038).The similar results were not been found in other hydroxylated metabolites as internal exposure group.Conclusion Increasing levels of urinary 1-hydroxypyrene might resulting in cell cycle of lymphocyte disorders,mainly for G0/G1 phase shorten and S phase arrest.
