Effect of fluorochloridone on oxidative stress in primary co-cultured sertoli-germ cell of rat
10.3760/cma.j.issn.1001-9391.2016.11.001
- VernacularTitle:氟咯草酮对大鼠原代培养支持-生精细胞氧化应激的影响
- Author:
Songlin WU
1
;
Leirui XU
;
Zhijun ZHOU
Author Information
1. 361008,厦门医学院医学技术系
- Keywords:
Fluorochloridone;
Rat;
Sertoli-germ cell;
Superoxide dismutase;
Gatalase
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(11):801-804
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effect of fluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism.Methods primary co-cultured sertoli-germ cell was made by two steps of enzyme digestion with SD rat testes,after 24h of Sertoli-germ cell isolation,A 0.1％ DMSO solvent control group and three FLC exposure groups (10-6、10-7、104 mol/L) were selected,cultured cell for 24h,then MTr assay and index detection of oxidative stress were performed.Results The mortality of primary co-cultured sertoli-germ cell exposedto 10-6mol/LFLC was significantly higher than controlgroupandFLC exposure groups (10-7、10-8mol/L) (P＜0.05).10-6mol/L FLC reduced enzyme activity of CAT,SOD and GSH.Px,depressed GSH level,elevated MDA level,and had significant difference than control group and FLC exposure groups (10-7、10-8 mol/L) (P＜ 0.05).10-7 mol/L FLC decreased enzyme activity of CAT than control group,depressed enzyme activity of SOD than 10-8 mol/L FLC,lowered GSH level than control group and FLC exposure groups (10-8 mol/L),and had statistical difference (P＜0.05).Conclusion FLC can damage primary co-cultured sertoli-germ cell of rat,its possible mechanism is relevant to oxidative stress.
