Transcriptome Comparison of Susceptible and Resistant Wheat in Response to Powdery Mildew Infection
10.1016/j.gpb.2012.05.002
- Author:
Xin MINGMING
1
,
2
,
3
;
Wang XIANGFENG
;
Peng HUIRU
;
Yao YINGYIN
;
Xie CHAOJIE
;
Han YAO
;
Ni ZHONGFU
;
Sun QIXIN
Author Information
1. State Key Laboratory for Agrobiotechnology and MOE Key Laboratory of Crop Heterosis and Utilization and MOA Key Laboratory of Crop Genomics and Genetic Improvement, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
2. National Plant Gene Research Centre(Beijing), Beijing 100094, China
3. School of Plant Sciences, University of Arizona, Tucson, AZ 85
- Keywords:
Wheat;
Pathogen resistance;
Powdery mildew;
Microarrays
- From:
Genomics, Proteomics & Bioinformatics
2012;10(2):94-106
- CountryChina
- Language:Chinese
-
Abstract:
Powdery mildew (Pm) caused by the infection of Blumeria graminis f.sp.tritici (Bgt) is a worldwide crop disease resulting in significant loss of wheat yield.To profile the genes and pathways responding to the Bgt infection,here,using Affymetrix wheat microarrays,we compared the leaf transcriptomes before and after Bgt inoculation in two wheat genotypes,a Pm-susceptible cultivar Jingdong 8 (S) and its near-isogenic line (R) carrying a single Pm resistant gene Pm30.Our analysis showed that the original gene expression status in the S and R genotypes of wheat was almost identical before Bgt inoculation,since only 60 genes exhibited differential expression by P =0.01 cutoff.However,12 h after Bgt inoculation,3014 and 2800 genes in the S and R genotype,respectively,responded to infection.A wide range of pathways were involved,including cell wall fortification,flavonoid biosynthesis and metabolic processes.Furthermore,for the first time,we show that sense-antisense pair genes might be participants in wheat-powdery mildew interaction.In addition,the results of qRT-PCR analysis on several candidate genes were consistent with the microarray data in their expression patterns.In summary,this study reveals leaf transcriptome changes before and after powdery mildew infection in wheat near-isogenic lines,suggesting that powdery mildew resistance is a highly complex systematic response involving a large amount of gene regulation.
