Role of long-range regulatory element mbRin transcriptional regulation of apoptosis-related genes in apoptosis process participated with CDP

VernacularTitle:CDP参与凋亡过程中远程调控元件mbr对于凋亡相关基因的转录调控作用
Author: Jiajia JIANG ¹ ; Yin YANG ; Yujie SUN
Author Information

1. 南京医科大学
Keywords: long-range regulatory element mbr; BCL2; NOXA; CDP
From: Journal of Clinical Medicine in Practice 2017;21(9):1-5,15
CountryChina
Language:Chinese
Abstract: Objective To investigate whetheRCDP can replace SATB1 in BCL2 and NOXA transcription afteRSATB1 degradation during apoptosis.Methods CDP was over-expressed in Jurkat cells,and the transcription levels of BCL2 gene and NOXA gene were determined by real-time PCR.EMSA assay was used to detect the protein complex change in mbRand NOXA promoter.Results In the Jurkat cells,the SATB1 protein was present on the mbRelement and NOXA-SBS1.When the SATB1 and CDP ratio changed,the SATB1 protein complex disappeared and replaced with the CDP protein complex on mbRelement.CDP was able to promote the transcription of NOXA and inhibit the transcription of BCL2.Conclusion The ratio of SATB1 protein to its homologous protein CDP is critical foRsynergistic expression of BCL2 and NOXA genes mediated by the mbRlong distance regulation.