The expression of serum miRNAs in pneumoconiosis complicated with pulmonary tuberculosis patients
10.3760/cma.j.issn.1001-9391.2016.07.012
- VernacularTitle:尘肺并发肺结核患者血清miRNAs表达
- Author:
Yitao LIU
1
;
Qiang ZENG
;
Shuxin LI
;
Deyi YANG
;
Weiyi XING
;
Linmin FENG
;
Ming ZHANG
Author Information
1. 300011,天津市疾病预防控制中心
- Keywords:
Silicotuberculosis;
MiRNAs;
Serum;
Gene expression
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(7):525-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective To inquiry the changes of serum miRNAs expression in pneumoconiosis complicated with pulmonary tuberculosis patients.Methods The study subjects (all subjects are male) were divided into three groups:I stage pneumoconiosis (38 cases)、Pneumoconiosis complicated with pulmonary tuberculosis (12 cases) and healthy control (40cases).The first two groups come from Tianjin worker's sanatorium and healthy control group came from a Hospital Health Screening Center.RT-qPCR was used to detect the expression levels of miR-21,miR-200c,miR-16,miR-204,miR-206,miR-155,let-7g,miR-30b,miR-192,miR-29a in serum.Results Compared with the healthy control group,the expression levels of miR-204,miR-206,miR-21,miR-16,miR-29a,miR-155,miR-200c in other two groups are different (P＜0.05);Compared with the I stage pneumoconiosis,the expression levels of miR-155 is significantly higher (P＜0.05) and miR-29a is significantly lower (P＜0.05).Conclusion Compared with the I stage pneumoconiosis,the expression levels of miR-155 and miR-29a are significantly different,suggesting that these two miRNAs may play a important role in the process of pneumoconiosis complicated with pulmonary tuberculosis
