The role of BDNF pathway in lambda-cyhalothrin disrupting the promotion of 17β-Estradiol on Post-synaptic Density 95 protein expression in HT22 cell
10.3760/cma.j.issn.1001-9391.2016.07.003
- VernacularTitle:BDNF通路在功夫菊酯干扰雌激素促海马神经细胞PSD95表达中的作用
- Author:
Nian LI
1
;
Qunan WANG
;
Daji WU
;
Chengwei YANG
;
Binbin LUO
Author Information
1. 安徽医科大学公共卫生学院卫生毒理学教研室
- Keywords:
Lambda-cyhalothrin;
17β-Estradiol;
Brain Derived Neurotrophic Factor;
Post-synaptic Density 95
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(7):489-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of BDNF pathway on lambda-cyhalothrin interfering estrogen promoting the expression of PSD95 in hippocampus neurons.Methods HT22 cell line were used to,treating with lambda-cyhalothrin (LCT,50 μmol/L),17 β-Estradiol (E2,10 nmol/L),LCT (50 μmol/L) +TrkB FC (20 μg/ml),E2 (10 nmol/L) +TrkB FC (20 μg/ml),LCT(50 μmol/L) +ICI182 780 (1 μmol/L),E2 (10 nmol/L)+ ICI182 780 (1 μmol/L),LCT (50 μmol/L)+E2 (10 nmol/L) for 24 h.MTT kit was used to detect cell viability.Post-synaptic Density 95 protein expression was measured by western blot.ELISA assay was used to detect the level of brain derived neurotrophic factor (BDNF) of culture supernatant and cell.Results Campared to Sham,LCT or E2 could promote the expression of PSD95 LCT+ICI could reduce the expresion of BDNF (P＜0.05),campared to LCT,LCT+TrkB FC could reduce the expression of PSD95 and LCT+ICI cound reduce the expresion of BDNF (P＜0.05),campared to E2,E2+TrkB FC could reduce the expression of PSD95 and E2+ICI could reduce the expression of BDNF (P＜0.05),campared to E2,LCT+ E2 could reduce the expression of PSD95 and BDNF (P＜ 0.05).Conclusion BDNF pathway plays a key role in E2 promoting the expression of PSD95 in neural cells.Although LCT alone has a similar effect on E2.LCT could disrupt the promotion of E2 on PSD95 expression via BDNF pathway.
