Effects of forsythia suspense on the liver gene expression levels of rats with sepsis model
10.3760/cma.j.issn.1001-9391.2016.05.009
- VernacularTitle:连翘提取物对脓毒症大鼠肝基因表达的影响
- Author:
Dongqiang WANG
1
;
Pingping ZHANG
;
Xuejun SONG
;
Ying LIU
;
Yahui LIU
;
Zhijun LI
Author Information
1. 300192,天津市第一中心医院
- Keywords:
Plants,medicinal;
For SYTHIA SUSPENSA;
Sepsis;
Gene expression
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(5):352-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of forsythia extract on the liver gene expression levels of rats with sepsis model.Methods The 90 Wistar rats were randomly divided into three groups:sham operation group (n=30),sepsis model group (n=30) and forsythia group (n=30).The survival rates at 48 b and 72 h were observed for all groups.The sepsis model and forsythia group rats were prepared by "CLP" method.72 h later the rats were sacrificed by removed the vertebra.Under sterile conditions,cut the size of about 10 mm×10 mm×3mm rat liver tissue and placed in liquid nitrogen for use.The same with the sham operation group.The gene expression levels of livers in all groups were detected by the Applications Rat Genome 230 2.0 microarray,and the relative strength of both the fluorescence signal ratio＞2 or ＜-2 screening significantly different genes,by the US National Center for Biotechnology Information (NCBI) database query gene function and classify.Results Forsythia group 48 h,72 h rat mortality rates were 30％ and 50％,the sepsis model group 48 h,72 h rat mortality rates were 46.7％ and 70％,two groups 48 h,72 h mortality rates were compared,the differences were statistically significant (P＜0.05).72 hours after CLP,the genes with up-regulation in sepsis model group/sham operation group and with down-regulation in Forsythia group/sepsis model group were 14.The genes with downregulation in sepsis model group/sham operation group and with up-regulation in Forsythia group/sepsis model group were 11.The genes involves immune-related genes 8,metabolism genes 5,material transport two related genes,cell adhesion two related genes,cell proliferation,differentiation and apoptosis related genes 4,transcriptional regulation genes 2and other related gene.Conclusion Forsythia can reduce the 48,72 h mortality of rats with sepsis and can regulate abnormal sepsis liver genes which associated with tissue immunity,inflammation,metabolism occur regression expression.
