Phylogenetic Analysis of Brine Shrimp (Artemia) in China Using DNA Barcoding
- Author:
Wang WEIWEI
1
,
2
;
Luo QIBIN
;
Guo HAIYAN
;
Bossier PETER
;
Stappen Van GILBERT
;
Sorgeloos PATRICK
;
Xin NAIHONG
;
Sun QISHI
;
Hu SONGNIAN
;
Yu JUN
Author Information
1. Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China
2. Graduate University of Chinese Academy of Sciences, Beijing 100049,China
- Keywords:
Artemia;
DNA barcoding;
molecular phylogeny;
Tibetan Plateau
- From:
Genomics, Proteomics & Bioinformatics
2008;6(3):155-162
- CountryChina
- Language:Chinese
-
Abstract:
DNA barcoding is a powerful approach for characterizing species of organisms,especially those with almost identical morphological features, thereby helping to to establish phylogenetic relationships and reveal evolutionary histories. In this study, we chose a 648-bp segment of the mitochondrial gene, cytochrome c oxidase subunit 1 (COI), as a standard barcode region to establish phylogenetic relationships among brine shrimp (Artemia) species from major habitats around the world and further focused on the biodiversity of Artemia species in China, especially in the Tibetan Plateau. Samples from five major salt lakes of the Tibetan Plateau located at altitudes over 4,000 m showed clear differences from other Artemia populations in China. We also observed two consistent amino acid changes, 153A/V and 183L/F, in the COI gene between the high and low altitude species in China.Moreover, indels in the COI sequence were identified in cyst and adult samples unique to the Co Qen population from the Tibetan Plateau, demonstrating the need for additional investigations of the mitochondrial genome among Tibetan Artemia populations.
