Headspace solid-phase microextraction-gas chromatography for determination of 2,5-hexanedione in urine
10.3760/cma.j.issn.1001-9391.2015.04.022
- VernacularTitle:尿中2,5-己二酮测定的顶空固相微萃取气相色谱法
- Author:
Wei ZOU
1
;
Mengping ZHANG
;
Haiyan WEI
Author Information
1. 山东省职业卫生与职业病防治研究院
- Keywords:
2,5-Hexanedione;
Urine;
Headspace solid-phase microextraction;
Chromatography,gas
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2015;33(4):297-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of 2,5-hexanedione in urine by headspace solid-phase microextraction-gas chromatography.Methods After extraction by solid-phase microextraction head,2,5-hexanedione in urine was determined by gas chromatography and was quantified by external standard method.Results The concentration of 2,5-hexanedione in urine showed a linear relationship within the range of 0.1-20.0 μg/ml.The regression equation was y=261.36x-1.903 3,r=0.999 2.The minimum detectable concentration was 0.01 μg/ml.The recovery rate was 92.6％～97.1％,with a relative standard deviation (RSD) of 3.3％～5.8％.The intra-day and inter-day RSDs were 3.8％～6.2％ and 4.7％～6.3％ respectively.Conclusion This determination method has no requirement for organic solvents,features simple and rapid operation,possesses higher detection sensitivity,and applies well to the determination of 2,5-hexanedione in urine.
