Comparative Proteome Analysis of Breast Cancer and Adjacent Normal Breast Tissues in Human
- Author:
Deng SHI-SHAN
1
;
Xing TIAN-YONG
;
Zhou HONG-YING
;
Xiong RUO-HONG
;
Lu YOU-GUANG
;
Wen BIN
;
Liu SHANG-QING
;
Yang HUI-JUN
Author Information
1. 四川大学
- Keywords:
biomarker;
breast cancer;
2D-PAGE;
mass spectrometry;
molecular mechanism
- From:
Genomics, Proteomics & Bioinformatics
2006;4(3):165-172
- CountryChina
- Language:Chinese
-
Abstract:
Two-dimensional polyacryiamide gel electrophoresis (2D-PAGE) and matrixassisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS), incorporated with online database searching, were performed to investigate differential proteins of breast cancer and adjacent normal breast tissues. Considering that serum albumin is abundantly presented in normal control samples, 15 differential spots detected in 11 out of 12 (91.7%) breast cancer samples were identified by online SIENA-2DPAGE database searching and MALDI-TOF/TOF-MS analysis. The results indicate that pathological changes of breast cancer are concerned with augmentation of substance metabolism, promotion of proteolytic activity, decline of activity of some inhibitors of enzymes, and so on. Some important proteins involved in the pathological process of breast cancer with changed expression may be useful biomarkers, such as alpha-1-antitrypsin, EF1-beta, cathepsin D, TCTP, SMT3A, RPS12, and PSMA1, among which SMT3A,RPS12, and PSMA1 were first reported for breast cancer in this study.
