β-arrestin and NF-κB, AP-1 activity in peripheral blood mononuclear cells of guinea pigs sensitized by trichloroethylene
10.3760/cma.j.issn.1001-9391.2010.07.005
- VernacularTitle:三氯乙烯致敏豚鼠单核淋巴细胞中β-arrestin蛋白表达和核转录因子及激活蛋白-1活性的研究
- Author:
Li-Jie WANG
1
;
Rui-Juan GUO
;
Tong SHEN
;
Qi-Xing ZHU
Author Information
1. 安徽医科大学
- Keywords:
Trichloroethylene;
Sensitization,immunologic;
NF-κB
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2010;28(7):494-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulatory mechanism of immune response of guinea pigs sensitized by trichloroethylene (TCE), and the expression level of β-arrestin, and the activity of NF-κB and AP-1 in peripheral blood mononuclear cells (PBMC) of guinea pigs sensitized by TCE. Methods Guinea pigs were treated with TCE based on the guinea pig maximum response test (GPMT); Blank control group and DNCB positive control group were established. Scores of skin reaction were evaluated and used to determine whether or not allergy in guinea pig. Then TCE treated group was divided into sensitized group or un-sensitized group. The expression levels of β-arrestin protein, activity of NF-κB and AP-1 in PBMC were detected by Western Blotting and EMSA, respectively. TNF-α level in serum was detected by ELISA Kits. Results No erythema or edema was found in the control group; part of guinea pigs treated with TCE developed erythema and edema, while obvious erythema and edema could be found in DNCB group. The sensitization rates were 71.4% and 100% in TCE and DNCB group,respectively. Compared with TCE un-sensitized group, expression of β-arrestin and AP-1 activity were not significantly different in TCE sensitized group (P>0.05). While the NF-κB activity was elevated obviously(P<0.05). Compared with blank control groups [(32.118±12.550) pg/ml], serum TNF-α levels in TCE sensitized groups[(55.485±8.732) pg/ml] significantly elevated(P<0.05); Conclusion In guinea pigs, β-arrestin and AP-1 may not be activated, while the NF-κB activation is significant, and plays a immune regulatory role in the immune reaction of allergy induced by TCE .
