High-throughput Three-dimensional Gel Electrophoresis for Versatile Utilities: A Stacked Slice-gel System for Separation and Reactions (4SR)
- Author:
Salimullah MD.
1
;
Mori MASAKI
;
Nishigaki KOICHI
Author Information
1. Saitama University
- Keywords:
three-dimensional polyacrylamide gel electrophoresis (3D-PAGE);
high-throughput screening;
three-dimensional separation;
microarray technology
- From:
Genomics, Proteomics & Bioinformatics
2006;4(1):26-33
- CountryChina
- Language:Chinese
-
Abstract:
A novel high-throughput system, called the stacked slice-gel system for separation and reactions (4SR), was developed for the analysis of DNA/RNA and protein/peptide. The system provides a novel three-dimensional gel electrophoresis approach that exploits the property of stacked slice gels. It allows multiple samples simultaneously to react as well as to be separated, offering a two-dimensional (m×n) sample loading system. For this purpose, high-throughput multi-micro vessels (MMVs) containing variable numbers of wells (100 wells in this paper) have been used, which are made of 25 mm square-size polyacrylamide gels. Furthermore, after electrophoretic separation, a slice gel containing a desired sample can be easily removed and proceeded to the next step. Different biological reactions as well as successive separation of products were effectively carried out dealing with DNA/RNA and protein/peptide. It shows that this system has a diversity of potentials to be developed.
