Role of NEP1-40 in regulation of Wnt signaling pathway and regeneration of neural cells in neonatal rats with hypoxic ischemic encephalopathy

VernacularTitle:NEP1-40对缺氧缺血性脑病新生大鼠的Wnt信号通路胞增殖的调控作用
Author: Hua WANG ¹ ; Jingcheng WANG ; Yongxiang WANG ; Daxin WANG ; Yuping TAO ; Xinmin FENG ; Chuanzhi XIONG ; Jiaxiang GU ; Jinshan HE
Author Information

1. 江苏省苏北人民医院骨科
Keywords: hypoxic ischemic encephalopathy; Wnt signaling pathway; Nogo-A; NEP1-40; neural cell proliferation
From: Journal of Clinical Medicine in Practice 2017;21(5):1-4
CountryChina
Language:Chinese
Abstract: Objective To explore the role of Nogo-A receptor antagonist NEP1-40 in regulating regeneration of neural cells and related Wnt signaling pathway in neonatal rats with hypoxic ischemic encephalopathy (HIBD).Methods A total of 40 HIBD rats were divided into HIBD group and HIBD + NEP1-40 group,20 rats in each group.PCR Test,Western Blot Analysis,IHC test for cell proliferation and 8-isoprostane detection were used to evaluate regulation of NgR transcription factors in Wnt signaling pathway and proliferation of neural cells.Results The expressions of c-Jun and cMyc,at the protein level,were up-regulated after treatment with Nogo-A receptor antagonist NEP1-40 for 7 days,and the same change was observed at gene expression and Ki-67.There was no significant change of 8-isoprostane.Conclusion The c-Jun and c-Myc are the main transcription factors in Wnt signaling pathway by inhibition of NgR,and meanwhile the proliferation of nerve cells in subventricular zone increase.