Study on mitochondrial oxidative damage induced by hydro quinone/Cu2+ in mice bone marrow cells
10.3760/cma.j.issn.1001-9391.2001.01.018
- VernacularTitle:氢醌/Cu2+对小鼠骨髓细胞线粒体氧化损伤的研究
- Author:
Fanghong LI
1
;
Xingfen YANG
Author Information
1. Sun Yat-sen University of Medical Sciences
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2001;19(1):53-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study hydroquinone(HQ) induced mitochondrial oxidative injury in mice bone marrow cells in the presence of Cu 2+. Methods In the presence of 1 μmol/L Cu 2+,bone marrow cells isolated from mice were treated with 0,10,20 and 40 μmol/L HQ for 30 min for the dose-effect study,or treated with the combination of 1 μmol/L Cu2+ and 20 μmol/L HQ for 0,30,60 and 120 min for time -course study.Cell death rate(CDR),reactive oxygen species(ROS),mitochondrial e nzyme activity(MEA) and mitochondrial membrane potential(MMP) were observed. Results In the presence of 1 μmol/L Cu2+,the lowest dosage of HQ (10 μmol/L) and the shoytest exposure time(30 min,20 μmol/ L) caused increase of CDR(115% and 117% of control respectively) and ROS generat ion(374% and 541% of control respectively) as well as de crease of MEA(54% and 30% of control respectively) and MMP(67% and 71% of contro l respectively) in dose- and time-dependent manner(ROS:r=0.941,0.981;CDR: r=0.866,0.866;MEA:r=-0.842,-0.902,respectively).All data were statistica lly different when compared with the control(P<0.05).In addition,ROS appeare d to have a close relationship with CDR(r=0.943),MEA(r=-0.977) and M MP(r=-0.902). Conclusion In the presence of 1 μmol/L Cu2+,H Q could definitely cause mitochondrial oxidative injury in mice bone marrow cell s.Mitochondrial oxidative injury induced by ROS generation might be one of impor tant pathways leading to cytotoxicity of HQ.
