Protection of tea polyphenols(TPP) from damage induced by UVA in primary cultured rat skin keratinocytes
10.3760/cma.j.issn.1001-9391.2000.06.005
- VernacularTitle:长波紫外线对大鼠皮肤角朊细胞的损伤及茶多酚的保护作用
- Author:
Yicai FU
1
;
Xipeng JIN
;
Ying ZHANG
Author Information
1. Shanghai Medical University
- Keywords:
UVA;
Keratinocyte;
Cell culture;
Cell cycle;
Tea polyphenols
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2000;18(6):333-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of UVA and tea polyphenols(TPP,a kind of polyphenol substances from a special plant) on lipid peroxidation and cell kinetics in primarily cultured rat skin keratinocytes.Methods The release of cytoplasm enzyme, lactate dehydrogenase( LDH), lipid peroxidation product, malondialdehyde (MDA) ,and anti-peroxidant, glutathione peroxidase(GSH-Px) ,were assayed in primary cultured keratinocytes treated by UVA and TPP. Cytometry and Flow Cytometer were used to count the viable cells and the cell kinetics,too. Results UVA could obviously improve the release of LDH from 827.55 U/L to 1 312.47 U/L,increase the formation of peroxidation product MDA,decrease the level of GSH-Px and reduce the survival rate of cells and the cell proliferative index( PI) from 34.24% to 17.98 %. TPP could significantly protect and inhibit the damage induced by UVA. Conclusion UVA could damage skin keratinocytes, and TPP could protect keratinocytes from damage induced by UVA. The result could provide useful information in developing natural anti-violet product.
