Suicide Gene Therapy for Bladder Cancer Using a Recombinant Adenovirus Expressing Escherichia Coli Cytosine Deaminase.
- Author:
Miwon AHN
1
;
Ho Yeong LIM
;
Chinghai KAO
;
Thomas A GARDNER
;
Song Chu KO
;
Se Joong KIM
Author Information
1. Department of Urology, Ajou University School of Medicine, Suwon, Korea. sejoong@madang.ajou.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Bladder cancer;
Suicide gene therapy;
Cytosine deaminase;
Adenovirus
- MeSH:
Adenoviridae*;
Cell Culture Techniques;
Cell Line;
Cytosine Deaminase*;
Cytosine*;
Escherichia coli*;
Escherichia*;
Flucytosine;
Fluorouracil;
Genetic Therapy*;
Inhibitory Concentration 50;
Prognosis;
Rous sarcoma virus;
Suicide*;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
2003;44(3):244-249
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The poor prognosis of advanced bladder cancer requires the investigation of novel treatment modalities. In this study, we investigated the suicide gene therapy for bladder cancer, using the adenovirus-mediated expression of Escherichia coli cytosine deaminase (CD) in conjunction with the prodrug 5-fluorocytosine (5-FC). MATERIALS AND METHODS: A replication-deficient recombinant adenovirus, which contains the Rous sarcoma virus (RSV) promoter driving the expression of CD, (Ad-RSV-CD) was constructed. In vitro cell-killing assay, using Ad-RSV-CD (20 MOI) plus 5-FC (500muM), was performed in bladder cancer cell lines, HT-1376, UM-UC-3 and NBT-II. The CD enzymatic activity was measured in the Ad-RSV-CD (20 MOI) infected cells, and the concentrations of 5-fluorouracil (5-FU) yielding an IC50 were calculated for those cells. RESULTS: 5-FU dose response curve showed that IC50 of NBT-II was 0.8muM, HT-1376 1.0muM and UM-UC-3 5.1muM at day 6. The CD enzymatic activities of the Ad-RSV-CD infected UM-UC-3, HT-1376 and NBT-II cells were 5696, 4655, 1766 pmole/1x10(6) cells, respectively. Whereas the administration of 5-FC (500muM) or Ad-RSV-CD (20 MOI) alone demonstrated no cytotoxicity to cells, Ad-RSV-CD/5-FC exhibited a significant cytotoxic effect in the cells, especially the UM-UC-3 and HT-1376. CONCLUSIONS: Ad-RSV-CD/5-FC suicide gene therapy is effective for bladder cancer cells in cell cultures, suggesting this approach may have potential as a strategy for the treatment of bladder cancer.
