Differences in near-infrared fluorescence imaging and histological analysis of cheek mucosa in golden hamsters with different pathological states
10.7518/hxkq.2024.2024150
- VernacularTitle:金黄地鼠不同病理状态颊囊黏膜的近红外荧光成像差异分析
- Author:
Diya XIE
1
;
Danni SHAN
;
Lei ZHANG
;
Sheng CHEN
;
Yingyu NA
;
Zhiyong WANG
Author Information
1. 南京大学医学院附属口腔医院,南京市口腔医院,南京大学口腔医学研究所,南京 210008
- Keywords:
indocyanine green;
near-infrared fluorescence imaging;
oral potentially malignant disorders;
oral squamous cell carcinoma
- From:
West China Journal of Stomatology
2024;42(6):716-722
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore and analyze the feasibility of using indocyanine green(ICG)near-infrared fluores-cence(NIF)imaging technology for the early diagnosis of oral potential malignant disorders and oral squamous cell car-cinoma.Methods 7,12-Dimethylbenz[a]anthracene in acetone solution was used to induce various pathological models of buccal mucosal lesions(mild/moderate dysplasia,severe dysplasia,squamous cell carcinoma)in golden hamster.ICG-NIF was conducted for the quantitative analysis of the fluorescence signal of lesion tissue,and evaluation of the diagnos-tic and discriminative capabilities of the ICG-NIF technology for mucosal lesions in various pathological states.Immuno-histochemical staining was perform to examine the mi-crovessel density(MVD)and microlymphatic vessel den-sity(MLVD)of mucosa in various pathological states and explore the histological reasons underlying the differ-ences in fluorescence signals.Results The results of ICG-NIF fluorescence quantitative analysis reveal the higher fluorescence intensity of mucosal lesions in the experimental group compared with that of the normal mucosa on the control side,with statistical differences(P＜0.05).Moreover,the more severe the malignancy of mucosal lesions in the experimental group,the higher the fluorescence intensity.According to histopathological analysis,the malignant pro-gression of mucosal lesions in golden hamsters was accompanied with an increase in MVD(P＜0.05)and a decrease in MLVD(P＜0.05).Conclusion The abnormal proliferation of mucosal lesions in golden hamsters exhibits a difference in ICG-NIF fluorescence signal compared with normal mucosal tissue.Fluorescence quantitative analysis methods can provide assistance in differentiation and show potential for clinical applications.
