Protective effect of carvacrol hydrogel on the alveolar bone in rats with periodontitis
10.7518/hxkq.2024.2024037
- VernacularTitle:香芹酚水凝胶对牙周炎大鼠牙槽骨保护作用研究
- Author:
Lulu ZHOU
1
;
Nian TENG
;
Tiantian GAO
;
Hongbin WANG
;
Xiang GAO
Author Information
1. 东北农业大学动物医学学院,哈尔滨 150030
- Keywords:
periodontitis;
carvacrol;
hydrogel;
osteoprotegerin pathway;
nuclear transcription factor-κB pathway;
alveolar bone resorption
- From:
West China Journal of Stomatology
2024;42(5):593-608
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the protective effect and mechanism of carvacrol hydrogel on the alveolar bone in rats with periodontitis.Methods A thermosensitive hydrogel supported by carvacrol was pre-pared using poloxamer and hydroxypropyl methyl cellulose as matrix.SD rats were randomly divided into blank group,periodontitis group,blank hydrogel group,and low-,medium-,and high-dose hydrogel groups.The periodonti-tis symptoms and the CT structure of the alveolar bone were observed.The changes in liver,spleen,kidney,and peri-odontal tissues were observed.The related indexes of bone metabolism in serum were detected.The expression of os-teoprotegerin(OPG)and nuclear transcription factor-κB(NF-κB)pathway proteins was determined by Western blot.The levels of inflammatory factors were assessed by quantitative reverse transcription polymerase chain reaction(qRT-PCR).Results Carvacrol hydrogel had good slow release,biocompatibility,and cell adhesion.The periodontitis of rats in the carvacrol hydrogel group was significantly alleviated,the expression of OPG protein in gingival tissue was significantly increased(P<0.01),and the levels of receptor activator of NF-κB ligand(RANKL),receptor activator of NF-κB(RANK),NF-κB protein,and inflammatory factors were significantly decreased(P<0.01).Conclusion Car-vacrol hydrogel can regulate the OPG and NF-κB pathways,reduce alveolar bone absorption,and improve periodontal inflammation.
