Anti-inflammatory effects and related mechanisms of naringenin in human periodontal ligament stem cells under lipopolysaccharide stimulation based on RNA sequencing
10.7518/hxkq.2024.2023453
- VernacularTitle:基于转录组测序探讨柚皮素对脂多糖作用下人牙周膜干细胞的抗炎作用及相关机制
- Author:
Junyu LI
1
,
2
;
Xiaomei XU
;
Xingyu LIU
;
Ting ZENG
;
Li ZHANG
;
Qian ZHENG
Author Information
1. 西南医科大学附属口腔医院正畸科,泸州 646000
2. 口颌面修复重建和再生泸州市重点实验室,泸州 646000
- Keywords:
naringenin;
human periodontal ligament stem cells;
anti-inflammatory effect;
nuclear factor κB;
RNA sequencing
- From:
West China Journal of Stomatology
2024;42(4):512-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective RNA sequencing(RNA-seq)and bioinformatic analysis were combined and used to explore the anti-inflammatory effects and mechanisms of naringenin(Nar)in lipopolysaccharide(LPS)-stimulated human peri-odontal ligament stem cells(hPDLSCs).Methods Cell counting kit-8,quantitative real-time reverse transcription poly-merase chain reaction(qRT-PCR),and enzyme-linked immunosorbent assay(ELISA)were adopted to detect the effects of Nar on the proliferation and expression of inflammatory factors in LPS-stimulated hPDLSCs,screening for the opti-mal anti-inflammatory concentration of Nar.Differentially expressed genes(DEGs)were screened using|log2FC|≥1 and P≤0.05 as criteria.Volcano plot analysis,Kyoto En-cyclopedia of Genes and Genomes(KEGG)pathway en-richment analysis,the String database,and the MCODE module of Cytoscape were utilized to select core genes and enriched pathways.The effects on the nuclear factor κB(NF-κB)signaling pathway were verified using ELISA,qRT-PCR,and Western blot.Results Appropriate concentrations of Nar could alleviate the expression of inflammatory fac-tors and promote the proliferation of hPDLSCs stimulated by LPS.The best anti-inflammatory effect was achieved with 20 μmol/L Nar.RNA-seq showed significant enrichment of inflammation-related signaling pathways.The anti-inflamma-tory effect of Nar was mediated by inhibiting the NF-κB signaling pathway,similar to the effect of the NF-κB inhibitor BAY 11-7802.Conclusion Nar could exert its anti-inflammatory effects by inhibiting the NF-κB signaling pathway,making it a potential therapeutic option for the adjuvant treatment of periodontitis.
