Euphorbia helioscopia inhibits proliferation,invasion,and migration and promotes apoptosis of non-small cell lung cancer cells
10.12122/j.issn.1673-4254.2024.10.10
- VernacularTitle:中药泽漆抑制非小细胞肺癌细胞增殖、侵袭、迁移以及促进细胞凋亡
- Author:
Xuerou LIU
1
,
2
;
Yumei YANG
;
Wei LIU
;
Zhen ZHANG
;
Xingqi ZHOU
;
Wenyu XIE
;
Lin SHEN
;
Mengxiao ZHANG
;
Xian LI
;
Jialan ZANG
;
Shanshan LI
Author Information
1. 蚌埠医科大学药学院,安徽 蚌埠 233030
2. 安徽省生化药物工程技术研究中心,安徽 蚌埠 233030
- Keywords:
Euphorbia helioscopia;
non-small cell lung cancer;
proliferation;
apoptosis;
invasion and migration
- From:
Journal of Southern Medical University
2024;44(10):1918-1925
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Euphorbia helioscopia on biological behaviors of non-small cell lung cancer(NSCLC)cells.Methods NSCLC cell lines PC-9 and A549 treated with different concentrations of Euphorbia helioscopia preparations were examined for changes in proliferation,apoptosis,invasion and migration using CCK-8 assay,colony formation assay,flow cytometry,wound healing assay and Transwell assay.Western blotting was performed to detect the changes in protein expressions of Bax,Bcl-2,E-cadherin,vimentin,MMP2,and MMP9 in the treated cells.PC-9 cells were injected subcutaneously into BALB/C nude mice to establish a nude mouse subcutaneous tumor model.According to the growth of subcutaneous tumors,mice were randomly divided into control group:gavaged daily with saline;Euphorbia helioscopia-treated group:gavaged daily with Euphorbia helioscopia 65 mg/mL,and Euphorbia helioscopia granules were dissolved in saline;cisplatin-treated group:injected intraperitoneally with cisplatin 4 mg/kg every 5 days,6 mice per group.The subcutaneous tumor volume and mass changes of mice were measured,and the toxic effects of Euphorbia helioscopia on heart,liver,spleen,lung and kidney as well as the therapeutic effects of Euphorbia helioscopia were observed in the mice bearing tumor.Results Euphorbia helioscopia granules concentration-dependently inhibited the proliferation and survival of PC-9 and A549 cells,significantly promoted cell apoptosis,suppressed invasion and migration abilities of the cells,up-regulated the expression levels of E-cadherin and Bax,and down-regulated the expressions of Bcl-2,vimentin,MMP2,and MMP9.In the tumor-bearing mice,treatment with Euphorbia helioscopia significantly inhibited tumor growth without producing obvious toxicity in the vital organs.Conclusion Euphorbia helioscopia can inhibit proliferation,invasion,and migration and induces apoptosis of NSCLC cells in vitro.
