Tujia medicine Toddalia asiatica improves synovial pannus in rats with collagen-induced arthritis through the PI3K/Akt signaling pathway
10.12122/j.issn.1673-4254.2024.08.17
- VernacularTitle:三百棒通过调控PI3K/Akt信号通路改善胶原诱导性类风湿性关节炎大鼠的血管翳
- Author:
Shan XIANG
1
,
2
;
Zongxing ZHANG
;
Lu JIANG
;
Daozhong LIU
;
Weiyi LI
;
Zhuoma BAO
;
Rui TIAN
;
Dan CHENG
;
Lin YUAN
Author Information
1. 湖北恩施学院,湖北 恩施 445000
2. 湖北民族大学风湿性疾病发生与干预湖北省重点实验室,湖北 恩施 4450003
- Keywords:
Toddalia asiatica;
rheumatoid arthritis;
pannus;
inflammation;
PI3K/Akt signaling pathway
- From:
Journal of Southern Medical University
2024;44(8):1582-1588
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic mechanism of Tujia medicine Toddalia asiatica alcohol extract(TAAE)for synovial pannus formation in rats with college-induced arthritis(CIA).Methods Sixty male SD rats were randomized into normal control group,CIA model group,TGT group,3 TAAE treatment groups at low,medium and high doses(n=10).Except for those in the normal control group,all the rats were subjected to CIA modeling using a secondary immunization method and treatment with saline,TGT or TAAE by gavage once daily for 35 days.The severity of arthritis was assessed using arthritis index(AI)score,and knee joint synovium pathologies were examined with HE staining.Serum levels of TNF-α,IL-6,and IL-1β were detected with ELISA;the protein expressions of PI3K,Akt,p-PI3K,p-Akt,VEGF,endostatin,HIF-1α,MMP1,MMP3,and MMP9 in knee joint synovial tissues were determined using Western blotting,and the mRNA expressions of TNF-α,IL-6,IL-1β,VEGF,HIF-1α,PI3K,and Akt were detected with RT-PCR.Results Treatment of CIA rat models with TAAE and TGT significantly alleviated paw swelling,lowered AI scores,and reduced knee joint pathology,neoangiogenesis,and serum levels of inflammatory factors.TAAE treatment obviously increased endostatin protein expression,downregulated p-PI3K,p-Akt,MMP1,MMP3,MMP9,VEGF,and HIF-1α proteins,and reduced TNF-α,IL-6,IL-1β,PI3K,Akt,VEGF,and HIF-1α mRNA levels in the synovial tissues,and these changes were comparable between high-dose TAAE group and TGT group.Conclusion TAAE can improve joint symptoms and inhibit synovial pannus formation in CIA rats by regulating the expressions of HIF-1α,VEGF,endostatin,MMP1,MMP3,and MMP9 via the PI3K/Akt signalling pathway.
