Calcitonin gene-related peptide inhibits the expression of Nod-like receptor protein 3 to promote osteoblast differen-tiation in mouse osteoblasts in vitro
10.7518/hxkq.2016.01.003
- VernacularTitle:降钙素基因相关肽通过抑制Nod样受体蛋白3表达促进小鼠成骨细胞分化的研究
- Author:
Jun CAI
1
;
Jun LÜ
;
Shiting LI
;
Qiangguo GAO
;
Gang ZHANG
Author Information
1. 第三军医大学附属新桥医院口腔颌面外科
- Keywords:
Key words] calcitonin gene-related peptide;
Nod-like receptor protein 3;
interleukin-1β;
reactive oxygen species;
osteoblast
- From:
West China Journal of Stomatology
2016;(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to investigate the regulatory effects of calcitonin gene-related peptide (CGRP) on Nod-like receptor protein 3 (NLRP3) and interleukin-1β (IL-1β) to promote osteoblast differentiation. Methods Different concentrations of CGRP (0, 10, 30, 100 ng·mL-1) were added to mouse osteoblasts in vitro. The mRNA and protein expression levels of both NLRP3 and IL-1β were examined using Real-time polymerase chain reaction and Western blot, respectively. Moreover, the concentrations of IL-1β protein and intracellular reactive oxygen species (ROS) were detected using enzyme-linked immunosorbent assay and flow cytometry, respectively. The osteogenic differentiation of mouse osteoblasts was identified through alizarin red staining. Results The protein and mRNA expression levels of both NLRP3 and IL-1β significantly decreased (P<0.05) with increasing CGRP concentration. Moreover, the contents of intracellular ROS gradually decreased (P<0.05). The osteogenic differentiation of the osteoblasts was more enhanced in the group treated with 100 ng·mL-1 CGRP than in the empty group (0 ng·mL-1CGRP). Conclusion CGRP promotes osteoblast differentiation by inhibiting the expression of inflammatory factors.
