Expression of bone morphogenetic protein receptor 2 in cleft mouse embryonic palate induced by retinoic acid

VernacularTitle:骨形态发生蛋白受体2在维甲酸诱导腭裂小鼠胚胎腭突中的表达
Author: Mu CHEN ¹ ; Xue LIU ; Dongsheng YU ; Cheng WANG ; Weicai WANG ; Hongzhang HUANG
Author Information

1. 广东医学院附属南山医院口腔科
Keywords: bone morphogenetic protein receptor 2; retinoic acid; cleft palate
From: West China Journal of Stomatology 2015;(4):377-382
CountryChina
Language:Chinese
Abstract: Objective To?investigate?the?effects?of?all-trans?retinoic?acid?(atRA)?on?the?function?of?bone?morphogenetic?protein?receptor?2?(BMPR2)?expression?in?embryonic?palate.?Methods???Cleft?palate?mice?model?was?established?by?atRA.?On?gestation?day?(GD)?15?and?GD17,?the?pregnant?mice?were?killed?to?obtain?the?embryos?from?the?uteri.?The?embryonic?palates?were?stained?with?hematoxylin-eosin,?and?the?remaining?sections?were?used?for?the?immunohistochemistry?of?BMPR2?detection.?Reverse?transcription-polymerase?chain?reaction?was?performed?to?detect?the?expression?levels?of?Bmpr2?mRNA.?Results???In?the?atRA-treated?group,?short?extensions?and?failure?to?fuse?with?each?other?were?observed.?The?positive?expression?of?BMPR2?was?detected?in?developing?palatal?process?from?GD?15?to?GD?17?in?the?control?group.?Compared?with?those?of?the?control?group,?BMPR2?protein?and?Bmpr2?mRNA?decreased?in?the?atRA-treated?group?(P<0.05).?Conclusion???The?treatment?of?preg-nant?mice?with?retinoic?acid?produces?small?palatal?shelves?in?their?fetuses?and?down-regulates?BMPR2?expressions.