Study on lingual mucosa carcinogenesis of C57BL/6 mice induced by 4-nitroquinoline 1-oxide
10.7518/hxkq.2015.04.007
- VernacularTitle:4-硝基喹啉-1-氧化物诱导C57BL/6小鼠舌黏膜癌变的研究
- Author:
Xiaoming DAI
1
;
Hua LIU
;
Zhibin ZUO
;
Shaohua QIN
;
Yonghua RUAN
;
Yisong LI
Author Information
1. 昆明医科大学第一附属医院整形外科颌面组
- Keywords:
4-nitroquinoline 1-oxide;
C57BL/6 mice;
lingual mucosa;
carcinogenesis;
animal model
- From:
West China Journal of Stomatology
2015;(4):357-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective This?study?aimed?to?induce?carcinogenesis?of?lingual?mucosa?in?C57BL/6?mice?by?feeding?them?4-nitroquinoline?1-oxide?(4NQO)?solution.?Methods???A?total?of?85?C57BL/6?mice?were?randomly?divided?into?distilled?water?control?group?(DD?group,?n=5),?1,2-propylene?glycol?control?group?(PG?group,?n=5),?and?experimental?group?(EP?group,?n=75).?The?mice?in?the?experimental?group?were?medially?fed?in?15?cages.?By?contrast,?the?mice?in?DD,?EP,?and?PG?groups?were?watered with distilled water, 50 mg·L-1?4NQO?solution,?and?1,2-propylene?glycol?solution.?The?mice?in?EP?group?were?executed?every?two?weeks?from?week?0,?and?the?mice?in?the?control?groups?were?sacrificed?at?the?28th?week.?The?mice?were?weighed.?Mucosal?lesions?were?measured?by?macroscopic?observation?and?histopathologic?detection.?Results???One?mouse?in?EP?group?died?of?unknown?reason.?The?weight?of?the?mice?in?EP?group?presented?weight?loss?compared?with?the?mice?in?DD?and?PG?groups?after?the?24th?week.?Seventy-nine?macroscopic?lesions?were?observed?in?the?lingual?mucosa,?oral?floor,?and?upper?palatal?and?buccal?mucosa.?A?total?of?70?macroscopic?lesions?(88.6%)?were?located?in?the?lingual?mucosa.?Mucosal?lesions?changed?from?simple?hyperplasia?to?squamous?cell?carcinomas.?Well-differentiated?squamous?cell?carcinomas?were?observed?in?all?mice?of?EP?group?by?pathological?section?at?the?28th?week.?No?lesion?was?found?in?the?mice?of?DD?and?PG?groups.?Conclusion?The?animal?model?of?lingual?squamous?cell?carcinomas?was?successfully?established.?The?periods?from?12th?to?16th?week?and?20th?to?28th?week?were?the?ideal?times?for?the?research?on?pathogenesis?of?early?and?medial-advanced?stage?during?carcinogenesis?of?squamous?cell?carcinomas.
