Variations in protein concentration and albumin/globulin ratio of whole unstimulated saliva obtained from healthy ;people and patients with chronic periodontitis
- VernacularTitle:健康人与慢性牙周炎患者唾液蛋白质量浓度及白蛋白与球蛋白比值的变化
- Author:
Bomiao CUI
1
;
Yu YU
;
Wenwen YUAN
;
Wenjie ZHOU
;
Xuedong ZHOU
;
Ping ZHANG
Author Information
- Keywords: total protein of whole saliva; salivary albumin; salivary globulin; albumin/globulin ratio
- From: West China Journal of Stomatology 2015;(4):339-342
- CountryChina
- Language:Chinese
- Abstract: Objective We?detected?and?analyzed?the?clinical?values?of?total?protein?(TP),?albumin?(ALB),?globulin?(GLB),?and?ALB/GLB?ratio?(A/G)?of?whole?unstimulated?saliva?of?healthy?people?to?determine?the?time?of?day?when?saliva?composi-tion?is?relatively?stable.?We?compared?the?protein?concentration?and?A/G?of?whole?unstimulated?saliva?of?patients?with?chronic?periodontitis?with?those?of?healthy?volunteers?to?provide?references?for?diagnostic?methods?and?clinical?applications?of?saliva.?Methods???The?whole?saliva?of?37?healthy?subjects?were?collected?at?8:00,?9:30,?11:30,?13:00,?16:30,?and?21:00.?Meanwhile,?the?whole?saliva?of?24?patients?with?periodontitis?was?collected?in?the?morning.?Bicinchoninic?acid?method?was?used?to?detect?the?TP?content.?Saliva?ALB?was?detected?by?GF-D800?semi-automatic?biochemical?analyzer,?and?the?GLB?and?A/G?were?cal-culated.?Finally,?the?results?were?statistically?analyzed?using?SPSS?19.0.?Results???Salivary?protein?compositions?were?stable?in the morning on an empty stomach. Healthy people:TP, (1 354.35±389.52)μg·mL-1;ALB, (139.55±27.19)μg·mL-1;?GLB,?(1 211.80±360.73)μg·mL-1;A/G, 0.126 3±0.041 7. Subjects with chronic periodontitis:TP, (2 611.56±231.62)μg·mL-1;?ALB,?(296.27±17.34)μg·mL-1;GLB, (2 315.69±221.67)μg·mL-1;?A/G,?0.156?2±0.017?3.?The?contents?of?TP,?ALB,?and?GLB?in?healthy?individuals?at?different?periods?within?a?day?showed?significant?differences?(P<0.05),?which?were?mainly?reflected?in?the?levels?before?and?after?meals.?No?significant?difference?was?detected?in?A/G.?The?concentrations?of?TP,?ALB,?and?GLB?were?significantly?increased?in?patients?with?chronic?periodontitis?compared?with?those?in?healthy?volunteers.?However,?no?significant?difference?existed?in?A/G.?Conclusion???Salivary?protein?compositions?are?more?stable?in?the?morning?than?in?other?periods.?Thus,?mornings?can?be?set?as?the?time?of?specimen?collec-tion?in?future?research.?The?concentrations?of?TP,?ALB,?and?GLB?in?patients?with?chronic?periodontitis?are?higher?than?those?in?healthy?people.
