Interleukin-23 strengthens the anti-apoptotic and drug resistance of human tongue squamous cell carcinoma through the Wingless-related integration site/β-catenin pathway
10.7518/hxkq.2015.03.007
- VernacularTitle:白细胞介素-23通过无翅基因相关整合位点/β-连环蛋白通路增强舌鳞状细胞癌抗凋亡及耐药能力
- Author:
Qin YAN
1
;
Yuting SU
;
Yuepeng ZHOU
;
Haitao ZHU
;
Xihu YANG
;
Jianhui XU
Author Information
1. 江苏大学附属医院口腔科
- Keywords:
interleukin-23;
tongue squamous cell carci-noma;
drug resistance;
anti-apoptosis;
Wingless-related integration site
- From:
West China Journal of Stomatology
2015;(3):249-254
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis?study?aims?to?detect?the?expression?level?of?interleukin-23?(IL-23)?in?tongue?squamous?cell?carcinoma?tissues?and?its?relationship?with?clinical?prognosis,?as?well?as?explore?the?anti-apoptotic?and?drug?resistance?of?the?tongue?squamous?cell?line-SCC9?before?and?after?treatment?with?IL-23.?Methods???The?expression?of?IL-23?in?tumor?tissues?from?28?tongue?cancer?patients?was?analyzed?by?immunohistochemistry?assay.?Quantitative?real-time?polymerase?chain?reac-tion?(qRT-PCR)?was?used?to?detect?the?mRNA?expression?of?Wingless-related?integration?site?(Wnt)1?and?c-myc?in?SCC9?cells?treated with different IL-23 concentrations. After interferencing the β-catenin with small interfering RNA (siRNA), the expres-sion of β-catenin, B-cell lymphoma-2 (Bcl-2), ATP-binding cassette sub-family G member 2 (ABCG2), and permeability-glycoprotein?(P-gp)?in?SCC9?was?measured?by?Western?blot?analysis.?The?effect?of?IL-23?on?the?apoptotic?resistance?of?SCC9?to?cisplatin?was?examined?by?methyl?thiazolyl?tetrazolium?test.?Results???The?expression?of?IL-23?in?tongue?cancer?tissues?was?correlated?with?lymphatic?metastasis,?nerve?invasion,?and?the?recurrence?after?therapy?(P<0.05).?After?dealing?with?IL-23,?SCC9?showed?the?upregulation?effect?of?Bcl-2,?ABCG2?and?P-gp?expressions.?IL-23?was?closely?related?to?the?activation?level?of?the?Wnt?pathway?and?significantly?strengthened?the?resistance?to?cisplatin?(P<0.01).?Conclusion???IL-23?activates?the?Wnt?pathway?in?tongue?squamous?cell?carcinoma,?thereby?enhancing?its?resistance?to?apoptosis?and?drug.
