Immunoexpression and clinical significance of interleukin-21 and receptor activator of nuclear factor κB ligand in human periapical granulomas and radicular cysts
10.7518/hxkq.2015.03.006
- VernacularTitle:白细胞介素-21和核因子κB受体活化因子配体在人根尖囊肿和肉芽肿中的表达及临床意义
- Author:
Juhua HU
1
;
Qian LI
;
Yanqing WANG
;
Song LI
Author Information
1. 安徽医科大学口腔医学院·附属口腔医院
- Keywords:
interleukin-21;
receptor activator of nuclear factor κB ligand;
radicular cyst;
periapical granuloma
- From:
West China Journal of Stomatology
2015;(3):244-248
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis?study?aimed?to?detect?the?immunoexpression?of?interleukin-21?(IL-21)?and?receptor?activator?of?nuclear?factor κB ligand (RANKL) in periapical granulomas (PGs) and radicular cysts (RCs). The interaction of IL-21 with RANKL?and?its?role?in?periapical?pathogenesis?were?also?speculated.?Methods A total of 32 PGs and 23 RCs were selected as?experimental?samples.?Lesion?size?and?occurrence?of?tenderness?were?recorded.?Up?to?10?healthy?gingival?tissues?were?collected?as?normal?control?samples.?All?tissues?were?subjected?to?immunohistochemical?analysis?with?anti-human?IL-21?and?RANKL?polyclonal?antibodies.?The?correlations?of?IL-21?with?RANKL,?lesion?size,?and?the?occurrence?of?tenderness?of?the?PGs and RCs were evaluated.Results???IL-21-positive?cells?were?detected?in?all?periapical?lesion?tissues?but?not?in?normal?tissues.?In?the?cyst?group?and?granuloma?group,?the?corresponding?expression?levels?of?IL-21?were?59.92±6.57?and?36.80± 6.81,?whereas?those?of?RANKL?were?68.81±18.59?and?36.12±14.87,?respectively.?Moreover,?t-test?revealed?a?significantly?higher expression of IL-21 and RANKL in RCs than in PGs (P<0.05).?IL-21?and?RANKL?were?positively?correlated?in?both?PGs and RCs (P<0.05).?Furthermore,?IL-21?was?correlated?with?lesion?size?(P<0.05).?Conclusion???This?study?demonstrated?that?IL-21?is?potentially?involved?in?the?pathogenesis?of?apical?periodontitis?lesions.?A?role?in?the?exacerbation?of?chronic?inflammation,?as?well?as?in?bone?resorption,?is?suspected.?Further?studies?are?required?to?elucidate?the?specific?functions?of?IL-21?in?periradicular?inflammatory?processes.
