Experimental study on the effects of the nerve growth factor regulating calcitonin gene-related peptide in promoting the proliferation of MG-63in vitro
10.7518/hxkq.2015.03.004
- VernacularTitle:神经生长因子调控降钙素基因相关肽的表达及对MG-63细胞增殖的影响
- Author:
Song SUN
1
;
Qiangguo GAO
;
Gang ZHANG
;
Yinghui TAN
Author Information
1. 第三军医大学新桥医院口腔颌面外科
- Keywords:
nerve growth factor;
calcitonin gene-related peptide;
cell proliferation
- From:
West China Journal of Stomatology
2015;(3):234-237
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo?investigate?the?nerve?growth?factor?(NGF)?regulating?the?expression?of?calcitonin?gene-related?peptide?(CGRP)?in?promoting?the?proliferation?of?osteoblast-like?cell?(MG-63)?and?thus?illustrate?the?mechanism?of?the?NGF?in?wound?healing.?Methods???Different?concentrations?of?NGF?were?used?to?stimulate?MG-63.?The?expression?of?CGRP?was?detected?by?real-time?quantitative?polymerase?chain?reaction?(RT-QPCR)?and?enzyme-linked?immunosorbent?assay?after?1,?2,?3,?and?4?days.?The?proliferation?of?MG-63?was?detected?by?cell?counting?kit-8?(CCK-8).?The?expression?of?CGRP?mRNA?and?the?proliferation?of?MG-63?were?then?detected?by?RT-QPCR?and?CCK-8?after?adding?the?NGF?receptor?blocker.?Results???Com-pared?with?the?blank?control?group,?the?expression?of?CGRP?significantly?increased?by?stimulating?the?NGF.?The?expression?of?CGRP?was?positively?related?to?the?concentration?of?NGF?(P<0.05).?Moreover,?the?expression?of?CGRP?increased?by?pro-longing?the?NGF?stimulation?time.?The?proliferation?of?MG-63?increased?after?stimulating?the?NGF?(P<0.05).?After?adding?the?NGF?receptor?blocker,?the?expression?of?CGRP?and?the?proliferation?of?MG-63?correspondingly?decreased?(P<0.05).?Conclusion???NGF?can?up-regulate?the?expression?of?CGRP?and?increase?the?proliferation?of?MG-63.?Therefore,?NGF?plays?a?significant?role?in?wound?healing.
