Differential proteomics on synthetic antimicrobial decapeptide against Streptococcus mutans
10.7518/hxkq.2015.02.017
- VernacularTitle:十肽对变异链球菌作用的差异蛋白质组学研究
- Author:
Yi LIU
1
;
Wei FEI
;
Yanjun WANG
;
Yandong MU
;
Hongkun WU
Author Information
1. 四川省医学科学院·四川省人民医院口腔科
- Keywords:
decapeptide;
Streptococcus mutans;
differential proteomics
- From:
West China Journal of Stomatology
2015;(2):187-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective??To?compare?the?protein?profiles?between?decapeptide-treated?and?untreated?planktonic?cells?of?Streptococcus mutans?(S.mutans)?by?differential?proteomic?analysis?to?determine?and?identify?the?key?proteins.Methods??In?our?previous?study,?we?investigated?decapeptide?(KKVVFKVKFK–NH2),?which?was?a?novel?adenosine?monophosphate.?Com-pared?with?other?oral?pathogens?tested,?decapeptide?had?a?preferential?antibacterial?activity?against?S. mutans.?It?also?inhibited?S. mutans?biofilm?formation?and?reduced?the?one-day?developed?biofilm.?In?the?present?study,?we?first?synthesized?decapep-tide,?and?then?compared?the?protein?profiles?between?decapeptide-treated?and?untreated?planktonic?cells?of?S. mutans?by?two-dimensional?gel?electrophoresis?and?matrix-assisted?laser?desorption?ionization?time-of-flight?mass?spectrometry.?We?also?verified?different?expressions?of?key?protein?enolase?in?the?protein?level.?Results??The?results?showed?that?decapeptide?altered?the?protein?expression?of?planktonic?S. mutans.?These?proteins?were?functionally?involved?in?carbohydrate?degradation?by?gly-colysis,?protein?folding,?conjunction,?transport,?translation,?adenosine?triphosphate?binding,?protein?binding,?sequence-specific?DNA?binding,?transcription?factor?activity,?and?two-component?response?regulator?activity.?Western?blot?results?showed?that?enolase?protein?expression?decreased?obviously?in?decapeptide-treated?cells?of?S. mutans.?Conclusion??The?protein?expression?of?S. mutans?significantly?changed?after?synthetic?antimicrobial?decapeptide?treatment,?suggesting?that?decapeptide?may?pre-sent?a?preferential?effect?on?oral?caries?by?changing?the?expression?of?certain?key?proteins,?such?as?enolase?protein.
