Effects of anti-infection treatment on expressions of HLA-DR and CD86 in dendritic cells in rabbit buccal VX2 squa- mous cell carcinoma tissue with inflammation
10.7518/hxkq.2015.02.007
- VernacularTitle:抗感染对伴炎症兔颊VX2鳞状细胞癌组织中树突状细胞表面分子HLA-DR和CD86表达的影响
- Author:
Lin ZHANG
1
;
Nini ZHANG
;
Guilin HUANG
;
Xiaohua HU
;
Jie YI
;
Li YAO
Author Information
1. 遵义医学院附属口腔医院口腔颌面外科
- Keywords:
VX2 squamous cell carcinoma;
inflamma-tion;
antibiotic;
dendritic cell
- From:
West China Journal of Stomatology
2015;(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo?investigate?the?effects?of?anti-infection?treatment?on?the?expressions?of?antigen-presenting-related?membrane-surface?molecules?HLA-DR?and?CD86?in?dendritic?cells?(DCs)?in?rabbit?buccal?VX2?squamous?cell?carcinoma?tissue?complicated?with?local?inflammation.?Methods???Rabbit?buccal?VX2?squamous?cell?carcinoma?with?local?inflammation?models?that?were?established?by?inflammation?was?induced?by?inoculation?VX2?tumor,?mechanical?trauma,?and?drinking?of?milk?with?high?sugar?viscosity.?The?animals?were?divided?into?four?groups.?Group?A?(n=12):?rabbit?buccal?VX2?squamous?cell?carcinoma?with?local?inflammation,?procaine?penicillin?was?intramuscularly?given,?and?tinidazole?tablets?were?given?by?gavage?for?three?consecutive?days.?Group?B?(n=12): rabbit buccal VX2 squamous cell carcinoma with local inflammation, normal?saline?was?intramuscularly?given,?and?aspirin?were?given?by?gavage?for?three?consecutive?days.?Group?C?(n=12): rabbit buccal?VX2?squamous?cell?carcinoma?with?local?inflammation,?normal?saline?was?given?intramuscularly?and?by?gavage?for?three?consecutive?days.?Group?D?(n=10): rabbit buccal VX2 squamous cell carcinoma, normal saline was given intramuscu-larly?and?by?gavage?for?three?consecutive?days.?All?the?rabbits?were?sacrificed?for?collection?of?tumor?specimens,?and?the?expression?levels?of?membrane-surface?HLA-DR?and?CD86?in?DCs?of?tumor?specimens?were?detected?viaflow?cytometry.?Results???The?positive?expression?rate?of?HLA-DR?and?the?double?positive?expression?rate?of?HLA-DR?and?CD86?were?group?A>?group?D>?group?B>?group?C.?The?positive?expression?rate?of?CD86?were?group?A>?group?D>?group?B?and?group?C?(P<0.05).?Conclusion???Anti-infection?treatment?significantly?increased?the?expressions?of?HLA-DR?and?CD86?in?DCs?of?rabbit?buccal?VX2?squamous?cell?carcinoma?tissue?complicated?with?local?inflammation.
