Effects of concentrated growth factor extract on the proliferation and differentiation of MC3T3-E1 cells attached to titanium surfaces
10.7518/hxkq.2015.01.019
- VernacularTitle:浓缩生长因子提取液对钛片表面MC3T3-E1细胞增殖分化的影响
- Author:
Xin LI
1
;
Zhihong JIANG
;
Zhonghao LIU
Author Information
1. 烟台市口腔医院修复科
- Keywords:
concentrated growth factor;
osseointegration;
osteoblast
- From:
West China Journal of Stomatology
2015;(1):84-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of concentrated growth factor extract (CGFe) on the proliferation and differen-tiation of MC3T3-E1 osteoblasts attached to sandblasted and acid etched titanium surfaces. Methods Trials were divided into experimental and control groups. The experimental group used α-MEM that contained CGFe (10% FBS), whereas the control group only used α-MEM (10% FBS). MTT assay was employed to detect the number of osteoblasts on the first, third, and fifth days. Alkaline phosphatase (ALP) activity and scanning electron microscope (SEM) were used to detect the osteoblast differentiations on the third and fifth days and to observe the osteoblast extensions on titanium surfaces for 12 h, respectively. Meanwhile, the levels of the osteogenetic biomarkers Runt-related transcription factor-2 (Runx2) and Osterix (Osx) on the third and seventh days were quantified via real-time polymerase chain reaction (PCR). Results MTT assay indicated that on the first, third, and fifth days, the absorbance in the experimental group significantly increased than that in the control group (P<0.05). ALP activity: on the third and fifth days, the absorbance of the experimental group was significantly higher than that of the control group (P<0.05). SEM: at 12 h, the extension of the experimental group cells was larger than that of the control group. Real-time PCR: given the standardization in the group, the gene expression level of the control group on the third day was 1, and the Runx2 and Osx gene expressions in the experimental group were larger than those of the con-trol group. Conclusion CGFe can efficiently stimulate the proliferation, differentiation and extension of MC3T3-E1 cells.
