Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth
10.7518/hxkq.2015.01.017
- VernacularTitle:三氧化矿物凝聚体对乳、恒牙牙髓细胞增殖和分化影响的比较
- Author:
Minyong WANG
1
;
He LIU
;
Shenglin LI
;
Man QIN
Author Information
1. 北京大学口腔医学院·口腔医院儿童口腔科
- Keywords:
mineral trioxide aggregate;
calcium hydroxide;
alkaline phosphatase;
dentin sialophosphoprotein;
osteo-protegerin;
receptor activator of NF-κB ligand;
dental pulp cell
- From:
West China Journal of Stomatology
2015;(1):75-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth. Methods Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), and odontoclastogenesis-related factors, such as osteo-protegerin (OPG) and receptor activator of NF-κB ligand (RANKL), were determined by real time polymerase chain reac-tion (PCR). Results Primary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proli-feration rates than the control cells (P<0.01). By contrast, the MTA-treated group showed significantly higher proliferation rates than the control group (P<0.01). Real time PCR results showed that calcium hydroxide-treated primary pulp cells exhi-bited significantly decreased ALP, DSPP, and OPG expression compared with the control group (P<0.01). Conversely, the MTA-treated group displayed significantly increased ALP, DSPP, and OPG expression (P<0.01). Calcium hydroxide-treated primary pulp cells also exhibited significantly upregulated RANKL expression (P<0.01); by contrast, MTA-treated cells did not show any change in RANKL expression (P>0.05). Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression (P<0.01). However, the calcium hydroxide-treated group remained almost the same as the control group (P>0.05). Neither MTA nor calcium hydroxide affected OPG and RANKL expression in per-manent pulp cells (P>0.05). Conclusion MTA is more suitable as a pulp-capping agent, particularly in primary
teeth, than calcium hydroxide.
