Effects of hirudin on the expression of basic fibroblast growth factor and transforming growth factor-β1 in human gin-gival fibroblasts
10.7518/hxkq.2015.01.002
- VernacularTitle:水蛭素对牙龈成纤维细胞碱性成纤维细胞生长因子和转化生长因子-β1表达的影响
- Author:
Yi ZHENG
1
;
Kun XUAN
;
Lan NAN
;
Shuixue MO
Author Information
1. 广西医科大学附属口腔医院正畸科
- Keywords:
hirudin;
human gingival fibroblasts;
transforming growth factor-β1;
basic fibroblast growth factor
- From:
West China Journal of Stomatology
2015;(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the effects of hirudin on the expression of transforming growth factor (TGF-β1) and basic fibroblast growth factor (bFGF) in human gingival fibroblasts (HGFs) in vitro, as well to explore its func-tion in the mechanism of gingival remodeling. Methods After culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry. Results Compared with normal HGFs, TGF-β1 expres-sion promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P<0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P<0.05). Conclusion Orthodontic force may influence the balance of collagen synthesis and degradation in HGFs. Hirudin may modulate the balance of HGF collagen metabolism, thereby promoting gingival remodeling.
