Total saponins of Panax japonicus alleviates CCl4-induced acute liver injury in rats by regulating the PI3K/AktNF-κB signaling pathway
10.12122/j.issn.1673-4254.2024.02.06
- VernacularTitle:竹节参总皂苷缓解CCl4诱导的大鼠急性肝损伤:基于调控PI3K/Akt/NF-κB信号通路
- Author:
Guangyang WU
1
,
2
;
Tianli SONG
;
Lang TANG
;
Yiming WANG
;
Xu LIU
;
Sheng HUANG
Author Information
1. 湖北民族大学医学部,湖北 恩施 445000
2. 湖北恩施学院,湖北 恩施 445000
- Keywords:
acute liver injury;
total saponins of Panax japonicus;
PI3K/Akt/NF-κB;
anti-inflammation;
anti-oxidation
- From:
Journal of Southern Medical University
2024;44(2):244-251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of total saponins of Panax japonicus(TSPJ)against CCl4-induced acute liver injury(ALI)in rats and explore the underlying pharmacological mechanisms.Methods Male SD rat models of CCl4-induced ALI were given intraperitoneal injections of distilled water,100 mg/kg biphenyl bisabololol,or 50,100,and 200 mg/kg TSPJ during modeling(n=8).Liver functions(AST,ALT,TBil and ALP)of the rats were assessed and liver pathologies were observed with HE staining.Immunohistochemistry was used to detect the expressions of PI3K/Akt/NF-κB signaling pathway molecules in liver tissue;ELISA was used to determine the levels of T-SOD,GSH-Px,and MDA.Western blotting was performed to detect the expression levels of PI3K-Akt and SIRT6-NF-κB pathways in the liver tissue.Results Network pharmacological analysis indicated that the key pathways including PI3K/Akt mediated the therapeutic effect of TSPJ on ALI.In the rat models of ALI,treatments with biphenyl bisabololol and TSPJ significantly ameliorated CCl4-induced increase of serum levels AST,ALT,ALP,TBil and MDA and decrease of T-SOD and GSH-Px levels(all P<0.01).The rat models of ALI showed significantly increased expression of p-NF-κB(P<0.01),decreased expressions of PI3K,p-Akt and SIRT6 proteins,and elevated expression levels of p-NF-κB,TNF-α and IL-6 proteins in the liver,which were all significantly improved in the treatment groups(P<0.05 or 0.01).Conclusion TSPJ can effectively alleviate CCl4-induced ALI in rats by suppressing inflammatory responses and oxidative stress in the liver viaregulating the PI3K/Akt and SIRT6/NF-κB pathways.
