Cytotoxicity and antibacterial effect of silicone-based soft liner varnish containing Ag/TiO2:an in vitro study
10.7518/hxkq.2014.06.019
- VernacularTitle:添加载银纳米二氧化钛光亮漆体外细胞毒性及其抗菌性的实验研究
- Author:
Yongfa ZENG
1
;
Lianshui SHI
;
Qun DAI
;
Lin ZHANG
;
Ying KONG
Author Information
1. 南昌大学附属口腔医院综合科
- Keywords:
silicone-based soft liner;
varnish;
antibacterial agent;
Ag/TiO2;
Candida albicans
- From:
West China Journal of Stomatology
2014;(6):611-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective We aim to evaluate the cytotoxicity and antibacterial effects of Silagum-Comfort (SLC) siliconebased soft liner varnish containing Ag/TiO2 in vitro. Methods Silicone-based liner circular specimens, with a diameter of 10 mm and a thickness of 2 mm, were created and divided into six groups randomly. Ag/TiO2 was incorporated into the SLC varnish based on the agent/material weight rate of 0, 0.5%, 1.0%, 1.5%, 2.0%, and 2.5%. The mixed varnish was used to polish the SLC specimens. Antibacterial specimens (ATSLC) were created. Methyl thiazolyl diphenyl-tetrazolium bromide assay method was used to evaluate the 3T3 cell cytotoxicity of ATSLC in vitro. Surface roughness (Ra) measurements of ATSLC were obtained. The antibacterial effect of ATSLC on Candida albicans was examined using the pelliclesticking method. Results The cytotoxicity of ATSLC was graded from 0 to 2. The Ra of ATSLC increased when Ag/TiO2 agent concentration increased. However, the differences were not statistically significant compared with the 0 group. With increasing Ag/ TiO2 concentration, the antibacterial agent concentration and the antibacterial rate of ATSLC also increased. When the Ag/ TiO2 antibacterial agent concentration reached 2.0% and 2.5%, the antibacterial rate increased to 97.5% and 96.5, respectively. Conclusion Varnish containing Ag/TiO2 has excellent antibacterial effect and can significantly improve the antibacterial effect of silicone-based soft liner.
