Experimental Model of Intracerebral Hematoma in Rat.
- Author:
Se Hoon KIM
1
;
Jun Hyeok SONG
;
Hoon Kap LEE
;
Hye Sun KIM
;
Hung Seob CHUNG
;
Ki Chan LEE
Author Information
1. Department of Neurosurgery, College of Medicine, Korea University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Experimental model;
Intracerebral hematoma;
Rat
- MeSH:
Animals;
Basal Ganglia;
Blood-Brain Barrier;
Brain;
Brain Edema;
Brain Injuries;
Evans Blue;
Hematoma*;
Infusions, Intravenous;
Models, Animal;
Models, Theoretical*;
Mortality;
Needles;
Perfusion;
Rats*;
Rats, Sprague-Dawley
- From:Journal of Korean Neurosurgical Society
1996;25(1):40-51
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Spontaneous intracerebral hemorrhage(ICH) is one of the most common neurosurgical disorders associated with high morbidity and mortality. However, the treatment of the disease remains controversial. While aggressive removal of the blood clot is advocated by some, supportive care without clot removal is recommended by others. This dichotomy in therapeutic approaches undoubtedly derives from a poor understanding of the pathophysiological mechanisms that result in brain injury following intracerebral hematoma. Thus, effective animal model of intracerebral hematoma is necessary so that the injury mechanisms can be identified and the specific therapy developed. In an attempt to establish the highly reliable and reproducible experimental model of intracerebral hematoma, various amount(sham. 25microliter, 50microliter and 100microliter) of autologous blood was injected stereotactically into the left basal ganglia of the anesthetized rats. 16 Sprague-Dawley rats were divided into 4 groups. The sham operation and injections of 25microliter, 50microliter and 100microliter of autologous arterial blood were performed on each group. The animals were assessed for the size and pattern of the hematoma and the neurological outcome at 24 hours after the formation of intracerebral hematomas. The 25microliter-injection group showed the more reproducible size and shape of the hematoma confined to the basal ganglia with better neurological outcome, Additionally, the perihematomal ischemic zone was observed after intracardiac perfusion with 2% 2,3,5-triphenyltetrazolium chloride(TTC). Another 18 rats were assessed for the histopathological changes during the hyperacute stage of the intracerebral hematoma with Hematoxylin-Eosin staining. Finally another 24 rats were divided into 6 groups and injected with 25microliter of blood. They underwent intravenous infusion of Evans blue and were sacrificed at 1, 3, 6, 12, 24 and 72 hours after the formation intracerebral hematomas. The Evans blue extra asation was noted around the needle tract and hematoma that meant disruption of the blood-brain barrier. Significant increment of the water content with resultant brain edema was noted on the ipsilateral brain 6-12 hours after the formation of the intracerebral hematoma.
