Excessive fluoride inducing calcium overload and apoptosis of ameloblasts
10.7518/hxkq.2014.06.003
- VernacularTitle:过量氟诱导成釉细胞钙超载及细胞凋亡的实验研究
- Author:
Ying ZHANG
1
;
Lin MA
;
Jian LI
;
Ming ZHONG
;
Kaiqiang ZHANG
;
Hefeng GU
Author Information
1. 中国医科大学口腔医学院口腔预防教研室
- Keywords:
fluoride;
ameloblasts;
calcium overload;
calreticulin;
apoptosis
- From:
West China Journal of Stomatology
2014;(6):542-546
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of excessive fluoride on calcium overload and apoptosis in cultured rat ameloblasts in vitro. Methods Logarithmic-phase ameloblasts (HAT-7) were treated with 0, 0.4, 0.8, 1.6, 3.2, and 6.4 mmol·L-1 sodium fluoride (NaF) solution. Cell activities were detected by using a Cell Counting Kit 8 (CCK-8) assay after 48 h of treatment. The effect of fluoride on cell apoptosis was analyzed by using flow cytometry. Excessive fluoride-induced calcium concentration and calreticulin expression changes in ameloblasts were detected by using laser scanning confocal microscopy, Western blot analysis, and real-time quantitative polymerase chain reaction. Results NaF inhibited ameloblast activity at 1.6, 3.2, and 6.4 mmol·L?1 (dose-dependent) after 48 h of induction. The Ca2+ fluorescence intensity of HAT-7 cells incubated with 1.6 and 3.2 mmol·L?1 NaF was higher than that in the control group. The fluoride-induced early-stage apoptosis of ameloblasts after 48 h of induction and the early-stage apoptosis rate was positively correlated with fluoride concentration. Calreticulin mRNA expression in HAT-7 cells was higher than that in the control group after 48 h of incubation with 0.8, 1.2, and 1.6 mmol·L?1 NaF. Conclusion Excessive fluoride-induced calcium overload in ameloblasts and further caused endoplasmic reticulum stress-mediated apoptosis.
