In situ visual imaging of oral squamous cell carcinoma in mice by using near-infrared quantum dots conjugated with ;arginine-glycine-aspartic acid peptide fluorescent probes
10.7518/hxkq.2014.05.017
- VernacularTitle:近红外量子点连接的精氨酸-甘氨酸-天冬氨酸肽段荧光探针对口腔鳞状细胞癌的活体可视化成像
- Author:
Yunlong BAI
1
;
Hao HUANG
;
Kai YANG
;
Hong TANG
Author Information
1. 重庆医科大学附属第一医院口腔颌面外科
- Keywords:
oral squamous cell carcinoma;
quantum dots;
integrinαvβ3;
arginine-glycine-aspartic acid peptide;
imaging
- From:
West China Journal of Stomatology
2014;(5):498-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate in situ visualization using near-infrared quantum dots (QDs) conjugated with arginine-glycine-aspartic acid (RGD) peptide fluorescent probes in oral squamous cell carcinoma (OSCC). Methods QDs with emis-sion wavelength of 800 nm (QD800) were conjugated with RGD peptides to produce QD800-RGD fluorescent probes. Human OSCC cell line BcaCD885 was inoculated in nude mice cheeks to establish OSCC mouse models. Frozen BcaCD885 tumor slices were immunofluorescence double stained by using QD800-RGD and CD105 monoclonal antibody and were observed using a laser scanning confocal microscope. QD800-RGD was injected into the OSCC models through the tail veins, and the in situ visualization was analyzed at different time points. The mice were sacrificed 12 h after injection to isolate tumors for the ex vivo analysis of probe localization in the tumors. Results QD800-RGD specifically targeted the integrinαvβ3 expressed in the endothelial cells of tumor angiogenic vessels in vitro and in vivo, producing clear tumor fluorescence images after intravenous injection. The most complete tumor images with maximal signal-to-noise ratios were observed 0.5 h to 6 h after injection of the probe and significantly reduced 9 h after the injection. However, the tumor image was still clearly visible at 12 h. Conclusion Using intravenously injected QD800-RGD generates high quality OSCC images when integrinαvβ3, which is expressed in the endothelial cells of tumor angiogenic vessels, is used as the target. The technique offers great potential in the diagnosis and individual treatment of OSCC.
