Changes in expression of phospholipase C-gamma1tyr783 in young rat condylar cartilage during functional mandibular ;protraction
10.7518/hxkq.2014.05.007
- VernacularTitle:磷脂酶C-γ1tyr783在下颌功能前伸大鼠髁突软骨后部表达变化的研究
- Author:
Haiying SHENG
1
;
Yanping ZUO
;
Xiao CHEN
;
Xuewei YUAN
Author Information
1. 河北医科大学第一医院口腔正畸科
- Keywords:
functional mandibular protraction;
condylar cartilage;
phospholipase C-gammaltyr783;
bone remodeling
- From:
West China Journal of Stomatology
2014;(5):455-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the expression of phospholipase C-gammaltyr783 (PLC-γ1tyr783) in the condylar cartilage of a young rat during functional mandibular protraction. This work also explores the function of PLC-γ1tyr783 in the rat mandibular condylar cartilage bone remodeling, which could provide experimental evidence for clinical bone ortho-pedic work. Methods A total of 60 four-week-old male Sprague-Dawley (SD) rats were used in this study. The rats were divided equally and randomly into experimental group and control group. The functional appliances that were fitted to the upper incisors of the animals in the experimental group were worn 24 h a day after the rats were fed for 7 d with homemade pellet feed. The animals in the experimental group, along with their matched controls, were sacrificed after 1, 3, 7, 14, 21, and 28 d. The bilateral condylar was fixed, decalcified, dehyded, and then conventional paraffin embedded. Immunohisto-chemistry of PLC-γ1tyr783 was applied to observe its express distribution and variation. Results The expression of PLC-γ1tyr783 decreased gradually in the control group, which showed age-related changes (P>0.05). On the 14th day, PLC-γ1tyr783 expres-sion in the experimental group was significantly higher than that in the control group. PLC-γ1tyr783 expression began to appear statistically and significantly different between the two groups (P<0.01). Conclusion PLC-γ1tyr783 is involved in the bone remodeling process of the rat condylar cartilage after functional mandibular protraction.
