In vitro study of the effect of a lactoperoxidase-peroxidase-thiocyanate system with iodine on the cariogenicinity of Strep-tococcus mutans
10.7518/hxkq.2014.04.020
- VernacularTitle:含碘的乳过氧化物酶-过氧化氢-硫氰化物系统对变异链球菌致龋性的影响
- Author:
Xuejun LIU
1
;
Yao LIU
;
Jing LIANG
;
Lu SHI
;
Jinpu CHU
;
Beilei LI
Author Information
1. 郑州大学口腔医学院牙体牙髓病学教研室
- Keywords:
Streptococcus mutans;
lactoperoxidase;
iodine;
thiocyanate;
glucosyltransferase;
insoluble exopoly-saccharides
- From:
West China Journal of Stomatology
2014;(4):404-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the effect of a lactoperoxidase-peroxidase-thiocyanate (LPO-H2O2- SCN-) system with different concentrations of iodine (I-) on Streptococcus mutans (S. mutans), particularly on various parameters, including growth, adhesion, glucosyltransferase (GTF) enzyme activity, and insoluble exopolysaccharide synthesis. Methods S. mutans ATCC 25175 was used as experimental species. Clonal formation unit (CFU) were counted to investigate the inhibitory effect on bacterial growth. The inhibition rate of bacterial adherence was calculated to analyze the effect on adhesion. Anthrone method was used to determine the content of insoluble exopolysaccharides and the amount of reducing saccharides. GTF activity and enzyme activity were then determined. Results The inhibitory ability of the LPO-H2O2-SCN- system with I- on the cariogenicinity of S. mutans was strengthened as I- concentration was increased. At I- concentration≥100 μmol·L-1, the antibacterial effects were significantly increased compared with those of the control group (P<0.05). At I- concentration≥ 1 000 μmol·L-1, the antibacterial effects were significantly improved compared with those of the group with SCN- only (P< 0.05). At I- concentration≥100 μmol·L-1, the inhibition rate of bacterial adherence was>50%; insoluble exopolysaccharide synthesis and GTF enzyme activity were reduced (P<0.05). Conclusion The antibacterial effects of the LPO-H2O2-I- system were enhanced by adding I- to overcome the antagonistic effect of physiological SCN- concentration. LPO-H2O2-SCN- system with different concentrations of I- showed statistically significant inhibitory effects on growth, adhesion, insoluble exopolysaccharide synthesis, and GTF enzyme activity.
