Expressions of inflammatory and fibrogenic factors in perinephric and subcutaneous adipose tissues of patients with adrenocorticotropic hormone-independent Cushing's syndrome
10.3969/j.issn.1673-4254.2017.04.25
- VernacularTitle:促肾上腺皮质激素非依赖性库欣综合征患者肾周及皮下脂肪组织的炎症及纤维化
- Author:
Chunyan WU
1
;
Huijian ZHANG
;
Cunxia FAN
;
Peng WU
;
Qiang WEI
;
Yingying CAI
;
Shaozhou ZHOU
;
Ling WANG
;
Yaoming XUE
;
Meiping GUAN
Author Information
1. 南方医科大学南方医院内分泌代谢科
- Keywords:
Cushing's syndrome;
adipose tissues;
inflammation;
fibrosis
- From:
Journal of Southern Medical University
2017;37(4):563-566
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of inflammation- and fibrosis-related genes in perinephric and subcutaneous adipose tissues in patients with adrenocorticotropic hormone (ACTH)-independent Cushing's syndrome. Methods The perinephric and subcutaneous adipose tissues adipose tissues were obtained from 8 patients with ACTH-independent Cushing's syndrome undergoing laparoscopic retroperitoneal adrenalectomy. Real-time PCR was used to detect the mRNA expression levels of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), matrix metallopeptidase 2 (MMP-2), TIMP metallopeptidase inhibitor 1 (TIMP-1), early growth response 1 (EGR1), CCAAT/enhancer binding protein β(CEBPβ), uncoupling protein 1(UCP-1), PPARγ coactivator 1 alpha (PGC1α) and cell death-inducing DFFA-like effector a (CIDEA). Results The mRNA level of CIDEA was significantly higher in the perinephric adipose tissue (peri-N) than in the subcutaneous adipose tissue (subQ) (P<0.05). The expressions of CEBPβ, UCP-1, and PGC1αmRNA in the peri-N were similar with those in the subQ. The expressions of IL-6, TIMP1 and EGR1 mRNA in the subQ were significantly higher than those in the peri-N (P<0.05). No significant difference in TNF-α and MMP-2 mRNA levels was found between peri-N and subQ. Conclusion The expression levels of the inflammation-and fibrosis-related genes are higher in the subQ than in the peri-N of patients with ACTH-independent Cushing's syndrome, suggesting that chronic exposure to endogenous hypercortisolism may cause adipose tissue dysfunction.
