Effects of irradiation on proliferation and differentiation of MC3T3-E1 osteoblastic cells in vitro
10.7518/hxkq.2014.04.006
- VernacularTitle:辐射对体外培养MC3T3-E1成骨前体细胞增殖和分化的影响
- Author:
Yumei LI
1
;
Yimin ZHAO
;
Nianbao ZHA
;
Zhen SHU
;
Song ZHANG
Author Information
1. 襄阳市中心医院口腔科
- Keywords:
osteoblast;
gamma irradiation;
cell differentiation;
gene expression
- From:
West China Journal of Stomatology
2014;(4):341-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study is to investigate the effects of irradiation on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Methods MC3T3-E1 cells were irradiated 24 h after initial seeding. Gamma-radiation was administered at 0, 4, and 8 Gy as single doses by using a 60Co source. Cell proliferation was assessed at days 1, 3, 5, and 7 post-irradiation by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide assay. The collagen secretion of the cells was measured through sirius red staining at day 12 post-irradiation. The expressions of osteogenesis-related genes were assessed through real time fluorescence quantitative polymerase chain reaction at day 16 post-irradiation. The matrix mineralization caused by cells was evaluated through alizarin red staining at day 28 post-irradiation. Results The cells exposed to 4 Gy or 8 Gy demonstrated significantly lower proliferation rates compared with the non-irradiated group. Doses of 4 Gy or more significantly inhibited the expressions of osteogenesis-related genes (Osterix and osteocalcin). Collagen secretion and cell mineralization were significantly reduced by the 8 Gy dose. Conclusion 60Co γ-rays dose-dependently suppress the proliferation, collagen secretion, and mineralization of MC3T3-E1 cells. Furthermore, radiation seems to dose-dependently inhibit the expressions of osteogenesis-related genes of the cells.
